PXD038330-1

PXD038330 is an original dataset announced via ProteomeXchange.

Title	Infection-induced peripheral mitochondria fission drives ER encapsulations that rescue bioenergetics
Description	During infection, viruses rely on altering organelle shape, location, and abundance to modulate cellular functions. The prevalent pathogen human cytomegalovirus (HCMV) induces mitochondria fragmentation while paradoxically promoting respiration. Using super-resolution and cryo-electron tomography, we establish that HCMV infection induces peripheral mitochondria fission, the progeny of which form mitochondria-ER encapsulations (MENCs). Proteomics and metabolic assays demonstrate that MENC resident protein PTPIP51 promotes mitochondria health by regulating calcium transfer, membrane potential, and cellular respiration. MENCs stabilize pro-viral mitochondria-mitochondria contacts (MiMiCs) that facilitate the transfer of membrane potential and further increase respiration. Beyond infection, we show that metastatic melanoma cells, which exhibit similar fragmentation/respiration phenotypes, also form PTPIP51-enriched MENCs. Given the prevalence of mitochondria fragmentation in diverse disease states, MENCs can provide an explanation for uncharacterized facets of pathogenesis.
HostingRepository	PRIDE
AnnounceDate	2024-07-25
AnnouncementXML	Submission_2024-07-25_10:02:50.682.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD038330
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Will Hofstadter
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-23 04:55:50	ID requested
⏵ 1	2024-07-25 10:02:51	announced

10.6019/PXD038330;

submitter keyword: HCMV,Mitochondria, Virus, PTPIP51

William Hofstadter
contact affiliation	Princeton Univeristy, Department of Molecular Biology, Cristea Lab
contact email	wah2@princeton.edu
lab head
Will Hofstadter
contact affiliation	Princeton University
contact email	wah2@princeton.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD038330
     1. Label: PRIDE project
     2. Name: Infection-induced peripheral mitochondria fission drives ER encapsulations that rescue bioenergetics