PXD038307 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The identification of phosphorylation sites of IBTK by shotgun proteomics |
Description | Translation is a tightly regulated process, and the mTORC1-S6K signaling axis plays a critical role in this control. Binding of eIF4F to the cap is hindered by eIF4E binding proteins (4EBPs), which, when hypophosphorylated, sequester eIF4E and prevent its association with eIF4G. However, in response to positive stimuli such as growth factors, mitogens, and amino acids, mTORC1 phosphorylates 4EBPs and relieves this inhibition, allowing the formation of eIF4F and subsequent initiation of translation. We are interested to know whether IBTK-mediated eIF4A1 ubiquitination is regulated by mTORC1/S6K signaling. Indeed, quantitative phosphoproteomics studies revealed that several IBTK phosphorylation sites are markedly downregulated by treatment of mTOR inhibitor, Rapamycin or Torin 1. Thus, probable phosphorylation sites of IBTK were identified by MS analysis. |
HostingRepository | PRIDE |
AnnounceDate | 2024-05-10 |
AnnouncementXML | Submission_2024-05-10_07:05:55.857.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Huiru Sun |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-11-23 04:24:09 | ID requested | |
⏵ 1 | 2024-05-10 07:05:56 | announced | |
Publication List
Keyword List
submitter keyword: IBTK, phosphorylation, shotgun |
Contact List
Chenji Wang |
contact affiliation | Genetics Department, college of Life Sciences, Fudan University, China |
contact email | chenjiwang@fudan.edu.cn |
lab head | |
Huiru Sun |
contact affiliation | Fudan University |
contact email | 21112030011@m.fudan.edu.cn |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD038307
- Label: PRIDE project
- Name: The identification of phosphorylation sites of IBTK by shotgun proteomics