PXD038299-2

PXD038299 is an original dataset announced via ProteomeXchange.

Title	iTAG an optimized IMiD-induced degron for targeted protein degradation in human and murine cells
Description	To address the limitation associated with degron based systems, we have developed iTAG, a novel synthetic tag based on IMiDs/CELMoDs mechanism of action that improves and addresses the limitations of both PROTAC and previous IMiDs/CeLMoDs based tags. Using structural and sequence analysis, we systematically explored native and chimeric degron containing domains (DCDs) and evaluated their ability to induce degradation. We identified the optimal chimeric iTAG(DCD23 60aa) that elicits robust degradation of targets across cell types and subcellular localisations without exhibiting the well documented “hook effect” of PROTAC-based systems. We showed that iTAG can also induce target degradation by murine CRBN and enabled the exploration of natural neo-substrates that can be degraded by murine CRBN. Hence, the iTAG system constitutes a versatile tool to degrade targets across the human and murine proteome.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:01:43.677.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Fernando J. Sialana
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-23 01:38:32	ID requested
1	2023-06-27 12:39:42	announced
⏵ 2	2023-11-14 09:01:46	announced	2023-11-14: Updated project metadata.
3	2024-10-22 05:51:07	announced	2024-10-22: Updated project metadata.

Bouguenina H, Nicolaou S, Le Bihan YV, Bowling EA, Calderon C, Caldwell JJ, Harrington B, Hayes A, McAndrew PC, Mitsopoulos C, Sialana FJ, Scarpino A, Stubbs M, Thapaliya A, Tyagi S, Wang HZ, Wood F, Burke R, Raynaud F, Choudhary J, van Montfort RLM, Sadok A, Westbrook TF, Collins I, Chopra R, iTAG an optimized IMiD-induced degron for targeted protein degradation in human and murine cells. iScience, 26(7):107059(2023) [pubmed]

submitter keyword: LC-MSMS, iMiDs,targeted protein degradation, PROTACs

Jyoti S. Choudhary
contact affiliation	Institute of Cancer Research, London
contact email	jyoti.choudhary@icr.ac.uk
lab head
Fernando J. Sialana
contact affiliation	Institute of Cancer Research
contact email	fsialana@yahoo.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/06/PXD038299

PRIDE project URI

• PRIDE
  1. PXD038299
     1. Label: PRIDE project
     2. Name: iTAG an optimized IMiD-induced degron for targeted protein degradation in human and murine cells