PXD038286 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Define the interactome of IBTK by Affinity purification coupled with mass spectrometry (AP-MS) and proximity-dependent biotinylation identification (BioID) methods |
Description | Affinity purification coupled with mass spectrometry (AP-MS) and proximity-dependent biotinylation identification (BioID) methods are powerful tools to define the interactome for a specific protein bait. Whereas AP-MS results in the identification of proteins that are in a stable complex, BioID labels and identifies proteins that are in close proximity to the bait, resulting in overlapping yet distinct protein identifications. In order to comprehensively characterize the IBTK interactome networks in cells, we developed a tag workflow which allows for both AP-MS and BioID analysis with a single construct, pcDNA5/FRT vector containing FLAG-BirA-IBTK. |
HostingRepository | PRIDE |
AnnounceDate | 2024-05-10 |
AnnouncementXML | Submission_2024-05-10_07:07:30.838.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Huiru Sun |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | biotinylated residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-11-22 04:08:57 | ID requested | |
⏵ 1 | 2024-05-10 07:07:31 | announced | |
Publication List
Keyword List
submitter keyword: AP-MS,IBTK interactome, BioID |
Contact List
Chenji Wang |
contact affiliation | Genetics Department, college of Life Sciences, Fudan University, China |
contact email | chenjiwang@fudan.edu.cn |
lab head | |
Huiru Sun |
contact affiliation | Fudan University |
contact email | 21112030011@m.fudan.edu.cn |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD038286
- Label: PRIDE project
- Name: Define the interactome of IBTK by Affinity purification coupled with mass spectrometry (AP-MS) and proximity-dependent biotinylation identification (BioID) methods