PXD038264-2

PXD038264 is an original dataset announced via ProteomeXchange.

Title	The role of endosomal protein DENND10/FAM45A in extracellular vesicle release and cancer cell migration
Description	Cell migration is key to tumor metastasis, the most challenging clinical problem in cancer treatment. Here we are investigating the role of DENND10/FAM45A, a recently described endosomal protein, in cancer cell migration via modulating the release of extracellular vesicles (EVs). Bioinformatics data mining showed that high DENND10 expression is significantly associated with poor prognosis in multiple cancer types. DENND10 knockout (DENND10-KO) resulted in defective cell spreading and migration, with impaired formation of stress fibers and focal adhesions. EV secretion was reduced in DENND10-KO cells. Importantly, wild-type conditioned medium or EVs restored the migratory ability and cytoskeletal organization of DENND10-KO cells. Quantitative mass spectrometry revealed that DENND10 depletion led to distinct EV compositional profiles with remodeled constituents of adhesion molecules and extracellular matrix. Together, our study unveiled DENND10 as an intrinsic coordinator between EV release and cell migration, which could be exploited for designing new anti-cancer strategies.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:44:55.578.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yanling Zhang
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-22 02:44:43	ID requested
1	2024-06-14 00:58:09	announced
⏵ 2	2024-10-22 06:44:55	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: extracellular vesicle,Mouse, DENND10, mammary cancer, 4T1

Yanling Zhang
contact affiliation	Soochow University
contact email	yanlzhan@suda.edu.cn
lab head
Yanling Zhang
contact affiliation	Soochow University
contact email	yanlzhan@suda.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/06/PXD038264

PRIDE project URI

• PRIDE
  1. PXD038264
     1. Label: PRIDE project
     2. Name: The role of endosomal protein DENND10/FAM45A in extracellular vesicle release and cancer cell migration