PXD038245-3

PXD038245 is an original dataset announced via ProteomeXchange.

Title	Ultrasensitive Capillary Electrophoresis Trapped Ion Mobility Time-of-Flight Mass spectrometry Reveals Proteome Transition in the Brain’s Developing Circadian Pacemaker
Description	This project employed ultrasensitive proteomics in the discovery setting utilizing microanalytical capillary electrophoresis electrospray ionization (CE-ESI) and trapped ion mobility high-resolution mass spectrometry (TimsTOF HRMS) to quantify proteomic changes during development in the suprachiasmatic nucleus (SCN), before and after the onset of photoreceptor-dependent visual function. Results from this work highlight the maturation of oligodendrocyte myelination as well as extracellular matrix proteins with implications for critical-period plasticity. This work marks the first integration of ultrasensitive proteomics by CE-ESI TimsTOF HRMS with super-resolution STORM microscopy provide, expanding analytical resources to help better understand the brain and vision.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:13:06.139.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Peter Nemes
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Bruker Daltonics timsTOF series

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-21 16:42:11	ID requested
1	2023-10-24 18:14:06	announced
2	2023-11-14 09:08:32	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 06:13:06	announced	2024-10-22: Updated project metadata.

10.1021/ACS.ANALCHEM.3C01987;

submitter keyword: neuron, proteomics, neural development,Capillary electrophoresis, mass spectrometry, retinal ganglion cell, mouse, suprachiasmatic nucleus, visual system development.

Peter Nemes
contact affiliation	Associate Professor, University of Maryland College Park, MD 20742
contact email	Nemes@umd.edu
lab head
Peter Nemes
contact affiliation	University of Maryland, College Park
contact email	nemes@umd.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD038245

PRIDE project URI

• PRIDE
  1. PXD038245
     1. Label: PRIDE project
     2. Name: Ultrasensitive Capillary Electrophoresis Trapped Ion Mobility Time-of-Flight Mass spectrometry Reveals Proteome Transition in the Brain’s Developing Circadian Pacemaker