PXD038241-2

PXD038241 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteomic and proteomic analysis of early epileptogenesis in mice at 4 and 24 h post-status epilepticus
Description	The phosphorylation-based signalling and protein changes occurring in the early phases after a pathophysiological insult, like status epilepticus, have not been detailed. To this end, the hippocampi of mice treated with pilocarpine and diazepam were examined by mass spectrometry at 4 and 24 h post-status epilepticus at vast depth using the tandem mass tag 11-plex system. In the accompanying article, the results implicated posttranscriptional regulatory proteins as early targets of increased phosphorylation. Also, the major targets of decreased phosphorylation at 4 h and 24 h were a subset of post synaptic density scaffold proteins, ion channels and neurotransmitter receptors. Many proteins targeted by dephosphorylation at 4 h also had decreased protein abundance at 24 h, indicating a phosphatase-mediated weakening of synapses.
HostingRepository	PRIDE
AnnounceDate	2024-04-30
AnnouncementXML	Submission_2024-04-30_06:15:00.055.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD038241
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Mark Graham
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-21 16:18:16	ID requested
1	2024-02-21 22:45:41	announced
⏵ 2	2024-04-30 06:15:01	announced	2024-04-30: Updated project metadata.
3	2024-10-22 06:31:34	announced	2024-10-22: Updated project metadata.

10.1016/j.isci.2024.109534;

10.6019/PXD038241;

Hurtado Silva M, van Waardenberg AJ, Mostafa A, Schoch S, Dietrich D, Graham ME, Multiomics of early epileptogenesis in mice reveals phosphorylation and dephosphorylation-directed growth and synaptic weakening. iScience, 27(4):109534(2024) [pubmed]

submitter keyword: Epileptogenesis, phosphoproteome, epilepsy, hippocampus, phosphorylation, mouse, proteome, tandem mass tag, phospho-signalling

Mark Evan Graham
contact affiliation	Children's Medical Research Institute
contact email	mgraham@cmri.org.au
lab head
Mark Graham
contact affiliation	Children's Medical Research Institute
contact email	mgraham@cmri.org.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/02/PXD038241

PRIDE project URI

• PRIDE
  1. PXD038241
     1. Label: PRIDE project
     2. Name: Phosphoproteomic and proteomic analysis of early epileptogenesis in mice at 4 and 24 h post-status epilepticus