PXD038234-1

PXD038234 is an original dataset announced via ProteomeXchange.

Title	GIPC3 AP-MS/MS from chick inner ear extracts
Description	To understand how GIPC3 exerts its effects on cuticular plate dimensions, we examined the GIPC3 protein-interaction network in hair cells. We immunoaffinity purified GIPC3 from crosslinked chicken inner ear extracts that were enriched for stereocilia, but still contain large amounts of hair-cell cytoplasmic proteins (Morgan et al., 2016). We carried out two separate experiments, each with ~1000 chicken ears, where we stabilized protein complexes using primary amine-reactive homo-bifunctional N-hydroxysuccimide ester crosslinkers that are thiol-cleavable and hence reversible (Mattson et al., 1993). In one experiment, we used dithiobis(succinimidyl propionate) (DSP), a membrane-permeable crosslinker that crosslinks extracellular and intracellular complexes; in the other experiment, we used 3,3'-dithiobis(sulfosuccinimidyl propionate) (DTSSP), which is membrane impermeant and thus only stabilizes extracellular and transmembrane complexes. We prepared soluble extracts of crude, crosslinked stereocilia and used these fractions for identifying GIPC3-interacting proteins.
HostingRepository	PRIDE
AnnounceDate	2023-05-09
AnnouncementXML	Submission_2023-05-09_07:41:02.210.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	PeterBarr-Gillespie
SpeciesList	scientific name: Gallus gallus (Chicken); NCBI TaxID: 9031;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-21 16:10:08	ID requested
⏵ 1	2023-05-09 07:41:04	announced
2	2023-11-14 08:45:40	announced	2023-11-14: Updated project metadata.

Chatterjee P, Morgan CP, Krey JF, Benson C, Goldsmith J, Bateschell M, Ricci AJ, Barr-Gillespie PG, GIPC3 couples to MYO6 and PDZ domain proteins, and shapes the hair cell apical region. J Cell Sci, 136(10):(2023) [pubmed]

submitter keyword: Stereocilia, myosin, cytoskeleton, hair cell, actin, hair bundle

PeterBarr-Gillespie
contact affiliation	Oregon Hearing Research Center Oregon Health & Science University Portland, OR 97239
contact email	gillespp@ohsu.edu
lab head
PeterBarr-Gillespie
contact affiliation	Oregon Hearing Research Center
contact email	gillespp@ohsu.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD038234
     1. Label: PRIDE project
     2. Name: GIPC3 AP-MS/MS from chick inner ear extracts