PXD038220-2

PXD038220 is an original dataset announced via ProteomeXchange.

Title	In Depth Characterization of Protein N-Glycosylation for a COVID-19 Variant-Design Vaccine Spike Protein
Description	In this work, we performed comprehensive glycosylation analysis to a candidate vaccine Spike protein. The Spike protein studied here was featured by a set of structural designs such as the six-proline substitution, furin cleavage site replacement, trimeric foldon incorporation as well as “hotspots” of SARS-CoV-2 variants. Besides, it was expressed in Chinese Hamster Ovary (CHO) Cell lines and manufactured at pilot scales, and thus, well represented latest variant-design vaccine of COVID-19. A combination of LC-MS/MS based analytical methods was dedicatedly applied to fully unravel the Spike N-glycosylation. Utilization of different protease was firstly explored and discussed. As a result, RPLC-MS/MS following Trypsin and GluC enzyme plus PNGase F treatment proved an effective and complementary method in analyzing N-glycosites and site-specific glycan. An HILIC-LC-MS based analytical method together with 2-AB labeling was also utilized to analyze N-glycan abundance for the Spike protein.
HostingRepository	iProX
AnnounceDate	2022-11-19
AnnouncementXML	Submission_2023-01-29_19:51:02.930.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jiangming Huang
SpeciesList	scientific name: Cricetulus griseus; NCBI TaxID: 10029;
ModificationList	N-glycosylated residue; 2-pyrrolidone-5-carboxylic acid (Glu); deamidated residue; S-carboxamidomethyl-L-cysteine
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-20 22:29:15	ID requested
1	2023-01-03 01:48:30	announced
⏵ 2	2023-01-29 19:51:03	announced	2023-01-30: Update publication information.

Huang J, Hou S, An J, Zhou C, In-depth characterization of protein N-glycosylation for a COVID-19 variant-design vaccine spike protein. Anal Bioanal Chem, 415(8):1455-1464(2023) [pubmed]

submitter keyword: COVID-19, Variant, Spike Glycosylation, LC-MSMS

Chenliang Zhou
contact affiliation	Shanghai Zerun Biotechnology Co., Ltd
contact email	zhouchenliang@walvax.com
lab head
Jiangming Huang
contact affiliation	Shanghai Zerun Biotechnology Co., Ltd
contact email	12110220006@fudan.edu.cn
dataset submitter

iProX dataset URI