PXD038198-2

PXD038198 is an original dataset announced via ProteomeXchange.

Title	Vitreous proteome of transgenic INSC94Y pigs compared to wild type
Description	INSC94Y transgenic pigs serve as a valuable model for permanent neonatal diabetes mellitus. Through genetically engineered modifications in the insulin (INS) gene, this model presents with impaired insulin secretion, resulting elevated fasting blood-glucose levels (hyperglycemia) [1].One of the severe complications of diabetes mellitus are hypoglycemia-mediated morphological abnormalities in the retina, also described as diabetic retinopathy (DR). Adjacent to the retina lies the vitreous, a gelatinous, highly hydrated matrix, which is important for ocular function and retinal physiology. It contains variety of proteins and signaling molecules, which are useful for the characterization of the biological activity of the vitreous itself, and may help to elucidate molecular processes occurring in the retina, especially under pathophysiological conditions and in disease. To gain insight into the proteomic profile of porcine vitreous and detect possible differences relevant to DR pathogenesis, we used discovery proteomics for analysis of INSC94Y vitreous compared to wild-type controls. Reference: [1] Renner, S.; Braun-Reichhart, C.; Blutke, A.; Herbach, N.; Emrich, D.; Streckel, E.; Wunsch, A.; Kessler, B.; Kurome, M.; Bahr, A.; et al. Permanent neonatal diabetes in INS(C94Y) transgenic pigs. Diabetes 2013, 62, 1505-1511, doi:10.2337/db12-1065.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:56:41.089.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Roxane Degroote
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-17 07:11:47	ID requested
1	2024-08-27 07:08:42	announced
⏵ 2	2024-10-22 06:56:41	announced	2024-10-22: Updated project metadata.

10.1002/PMIC.202300591;

submitter keyword: quantitative label-free LC-MS/MS

diabetes mellitus

permanent neonatal diabetes

diabetic retinopathy

genetically modified pig

INSC94Y

porcine model

vitreous

Stefanie M Hauck
contact affiliation	Research Unit Protein Science, Helmholtz Center Munich, German Research Center for Environmental Health GmbH, Germany
contact email	hauck@helmholtz-muenchen.de
lab head
Roxane Degroote
contact affiliation	LMU Munich, Veterinary Sciences, Chair of Physiology, Lena-Christ-Straße 48, 82152 Planegg/Martinsried, Germany
contact email	r.degroote@lmu.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD038198
     1. Label: PRIDE project
     2. Name: Vitreous proteome of transgenic INSC94Y pigs compared to wild type