PXD038115-1

PXD038115 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Oncogenic pathways in uveal melanoma identified by phosphoproteomic analysis
Description	The uveal melanoma cell lines MP41 and MP46 driven by oncogenic G11 (Q209L) and Gq (Q209L), respectively, and a control cell line OCM-1A driven by oncogenic BRAF (V600E) were treated for 24 hours with FR900359, a highly specific inhibitor of Gq/11. Protein extracts were collected and TMT labeled prior to LC-MS analysis. The enrichment of phosphopeptides from fractions of each sample was performed using IMAC prior to LC-MS. Proteomic and phospho-proteomic data analyses were performed to identify changes in post-translational modification in signaling pathways regulated by Gq/11 in these cells.
HostingRepository	MassIVE
AnnounceDate	2024-02-16
AnnouncementXML	Submission_2024-02-16_08:27:11.989.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jim Malone
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	unknown modification; unknown modification; Pyro-carbamidomethyl; Glu->pyro-Glu; Acetyl; Oxidation
Instrument	Q Exactive Plus

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-11 09:31:47	ID requested
⏵ 1	2024-02-16 08:27:12	announced

Publication List

Onken MD, Erdmann-Gilmore P, Zhang Q, Thapa K, King E, Kaltenbronn KM, Noda SE, Makepeace CM, Goldfarb D, Babur Ö, Townsend RR, Blumer KJ, Protein Kinase Signaling Networks Driven by Oncogenic Gq/11 in Uveal Melanoma Identified by Phosphoproteomic and Bioinformatic Analyses. Mol Cell Proteomics, 22(11):100649(2023) [pubmed]

Onken MD, Erdmann-Gilmore P, Zhang Q, Thapa K, King E, Kaltenbronn KM, Noda SE, Makepeace CM, Goldfarb D, Babur Ö, Townsend RR, Blumer KJ, Protein Kinase Signaling Networks Driven by Oncogenic Gq/11 in Uveal Melanoma Identified by Phosphoproteomic and Bioinformatic Analyses. Mol Cell Proteomics, 22(11):100649(2023) [pubmed]

Keyword List

submitter keyword: Phosphoproteomics, G-proteins, Kinases, Uveal melanoma, FR900359

submitter keyword: Phosphoproteomics, G-proteins, Kinases, Uveal melanoma, FR900359

Contact List

Kendall J. Blumer
contact affiliation	Washington University in St. Louis
contact email	kblumer@wustl.edu
lab head
Jim Malone
contact affiliation	Washington University Sch
contact email	jmalone@wustl.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v05/MSV000090700/