PXD038104-1

PXD038104 is an original dataset announced via ProteomeXchange.

Title	Bacterial host adaptation through sequence and structural variations of a single type III effector gene
Description	Molecular mechanisms underlying quantitative variations of pathogenicity remain elusive. Here, we identified the Xanthomonas campestris XopJ6 effector that triggers disease resistance in cauliflower and Arabidopsis thaliana. XopJ6 is a close homolog of the Ralstonia solanacearum PopP2 YopJ family acetyltransferase. XopJ6 is recognized by the RRS1-R/RPS4 NLR pair that integrates a WRKY decoy domain mimicking effector true targets. We identified a XopJ6 natural variant carrying a single residue substitution in XopJ6 WRKY-binding site that disrupts interaction with WRKY proteins. This mutation allows XopJ6 to evade immune perception while retaining XopJ6 virulence functions. Interestingly, xopJ6 resides in a Tn3-family transposon likely contributing to xopJ6 copy number variation (CNV). Using genetic complementation assays, we demonstrate that xopJ6 CNV allows fine-tuning of pathogen virulence on Arabidopsis through gene dosage-mediated modulation of xopJ6 expression. Together, our findings highlight how sequence and structural genetic variations restricted at a particular effector gene contribute to bacterial host adaptation.
HostingRepository	PRIDE
AnnounceDate	2024-01-29
AnnouncementXML	Submission_2024-01-29_08:07:57.159.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Carole Pichereaux
SpeciesList	scientific name: Ralstonia pseudosolanacearum; NCBI TaxID: 1310165;
ModificationList	acetylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-11 09:02:42	ID requested
⏵ 1	2024-01-29 08:07:57	announced

Dataset with its publication pending

submitter keyword: Arabidopsis, acetyltransferase, Ralstonia, immune receptor, popP2, copy number variation, cauliflower,Xanthomonas, xopJ6

Carole Pichereaux
contact affiliation	CNRS, FRAIB, IPBS, France , Toulouse
contact email	Carole.Pichereaux@ipbs.fr
lab head
Carole Pichereaux
contact affiliation	CNRS
contact email	Carole.pichereaux@ipbs.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD038104
     1. Label: PRIDE project
     2. Name: Bacterial host adaptation through sequence and structural variations of a single type III effector gene