PXD038038-1

PXD038038 is an original dataset announced via ProteomeXchange.

Title	Revealing Corynebacterium glutamicum proteoforms through top-down proteomics
Description	Corynebacterium glutamicum is a bacterium widely employed in the industrial production of amino acids as well as a broad range of other biotechnological products. The present study describes the characterization of C. glutamicum proteoforms, and their post-translational modifications (PTMs) employing top-down proteomics. Despite previous evidence of PTMs having roles in the regulation of C. glutamicum metabolism, this is the first top-down proteome analysis of this organism. We identified 1125 proteoforms from 273 proteins, with 60 % of proteins presenting at least one mass shift, suggesting the presence of PTMs, including several acetylated, oxidized and formylated proteoforms. Furthermore, proteins relevant to amino acid production, protein secretion, and oxidative stress were identified with mass shifts suggesting the presence of uncharacterized PTMs and proteoforms that may affect biotechnologically relevant processes in this industrial workhorse. For instance, the membrane proteins mepB and SecG were identified as a cleaved and a formylated proteoform, respectively. While in the central metabolism, OdhI was identified as two proteoforms with potential biological relevance: a cleaved proteoform and a proteoform with PTMs corresponding to a 70 Da mass shift.
HostingRepository	PRIDE
AnnounceDate	2022-12-10
AnnouncementXML	Submission_2022-12-10_05:13:13.536.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	ReynaldoMagalhaes Melo
SpeciesList	scientific name: Corynebacterium glutamicum ATCC 13032; NCBI TaxID: 196627;
ModificationList	acetylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-08 07:37:43	ID requested
⏵ 1	2022-12-10 05:13:13	announced

Dataset with its publication pending

submitter keyword: top-down proteomics, Corynebacterium glutamicum,Proteoforms

Marcelo Valle deSousa
contact affiliation	Laboratory of Protein Chemistry and Biochemistry, Department of Cell Biology, Institute of Biology, University of Brasilia, Brasilia, Brazil.
contact email	mvsousa@unb.br
lab head
ReynaldoMagalhaes Melo
contact affiliation	University of Brasilia
contact email	reynaldommelo@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD038038
     1. Label: PRIDE project
     2. Name: Revealing Corynebacterium glutamicum proteoforms through top-down proteomics