PXD037998-1

PXD037998 is an original dataset announced via ProteomeXchange.

Title	Identification of miR-16/29 targets in adipocytes by LC-MSMS
Description	Cancer cachexia is a multifactorial metabolic syndrome defined by the rapid loss of skeletal muscle mass and the loss of fat mass. Up 80% of cancer patients at the late stage with cachexia suffer from progressive atrophy of adipose tissue. Unlike studies on skeletal muscle wasting, there is limited research on fat loss in cachexia. It was noted that most patients suffer from fat loss as cancer progress. Fat loss precedes muscle loss, is associated with shorter survival, and is variable to timing and intensity in various cancer populations. Increased lipolysis may be the leading cause of fat loss in cancer cachexia. miRNAs are a class of non-coding RNAs of 19~25 nucleotides that regulate gene silencing by interacting with the 3’ untranslated region (UTR) of target mRNA to cause mRNA degradation and translational repression. miRNAs play multifaceted roles in pancreatic cancer proliferation, survival, metastasis, and chemoresistance. Aberrant expression of miRNA in circulating exosomes may play potential roles in modulating fat loss in cancer cachexia. We identified 2 miRNAs, miR-16 and miR-29, which have 2-fold higher expression existed in at PDAC cells. To explore which genes in adipogenesis and lipolysis were directly affected by miR-16-5p or/and miR-29a-3p, we analyzed the targets which were down-regulated in both miR-16-5p and miR-29a-3p-transfected 3T3-L1 cells by mass analysis.
HostingRepository	PRIDE
AnnounceDate	2024-06-13
AnnouncementXML	Submission_2024-06-13_10:25:58.629.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD037998
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Sui-Chih Tien
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	carbamoylated residue; acetylated residue; monohydroxylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-07 09:42:33	ID requested
⏵ 1	2024-06-13 10:25:59	announced
2	2024-10-22 06:44:51	announced	2024-10-22: Updated project metadata.

10.6019/PXD037998;

submitter keyword: LC-MSMS,Mouse, Adipocytes, miRNAs

Chun-Mei Hu
contact affiliation	Genomics Research Center, Academia Sinica, Taipei, Taiwan
contact email	CMHU1220@gate.sinica.edu.tw
lab head
Sui-Chih Tien
contact affiliation	Academic Sinica
contact email	suichihtien@gate.sinica.edu.tw
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD037998
     1. Label: PRIDE project
     2. Name: Identification of miR-16/29 targets in adipocytes by LC-MSMS