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PXD037995-1

PXD037995 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleFolding stabilities of ribosome-bound nascent polypeptides probed by mass spectrometry
DescriptionThe folding of most proteins occurs during the course of their translation while their tRNA-bound C-termini are embedded in the ribosome. How the close proximity of nascent proteins to the ribosome influences their folding thermodynamics remains poorly understood. Here, we have developed a mass spectrometry-based approach for determining the stabilities of nascent polypeptide chains using methionine oxidation as a folding probe. This approach enables quantitative measurements sub-global folding stabilities of ribosome nascent chains (RNCs) within complex protein mixtures and extracts. To validate the methodology, we analyzed the folding thermodynamics of three model proteins (DHFR, CheY and DinB) in soluble and ribosome-bound states. The data indicated that the ribosome can significantly alter the stability of nascent polypeptides. Ribosome-induced stability modulations were highly variable among different folding domains and were dependent on localized charge distributions within nascent polypeptides. The results implicated electrostatic interactions between the ribosome surface and nascent polypeptides as the cause of ribosome-induced stability modulations. The study establishes a robust proteomic methodology for analyzing localized stabilities within ribosome-bound nascent polypeptides and sheds light on how the ribosome influences the thermodynamics of protein folding.
HostingRepositoryPRIDE
AnnounceDate2023-08-09
AnnouncementXMLSubmission_2023-08-08_18:15:35.019.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterRuiyueTan
SpeciesList scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationListmonohydroxylated residue; formylated residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-11-07 09:27:57ID requested
12023-08-08 18:15:35announced
22023-11-14 06:41:28announced2023-11-14: Updated project metadata.
Publication List
Keyword List
submitter keyword: Protein stability
Co-translational protein folding
Ribosome
Translation
Proteomics
Mass spectrometry (MS)
Methionine oxidation
Contact List
SinaGhaemmaghami
contact affiliationGhaemmaghami lab, biology department, University of Rochester
contact emailsina.ghaemmaghami@rochester.edu
lab head
RuiyueTan
contact affiliationUniversity of Rochester
contact emailrtan12@ur.rochester.edu
dataset submitter
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Dataset FTP location
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