PXD037995 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Folding stabilities of ribosome-bound nascent polypeptides probed by mass spectrometry |
Description | The folding of most proteins occurs during the course of their translation while their tRNA-bound C-termini are embedded in the ribosome. How the close proximity of nascent proteins to the ribosome influences their folding thermodynamics remains poorly understood. Here, we have developed a mass spectrometry-based approach for determining the stabilities of nascent polypeptide chains using methionine oxidation as a folding probe. This approach enables quantitative measurements sub-global folding stabilities of ribosome nascent chains (RNCs) within complex protein mixtures and extracts. To validate the methodology, we analyzed the folding thermodynamics of three model proteins (DHFR, CheY and DinB) in soluble and ribosome-bound states. The data indicated that the ribosome can significantly alter the stability of nascent polypeptides. Ribosome-induced stability modulations were highly variable among different folding domains and were dependent on localized charge distributions within nascent polypeptides. The results implicated electrostatic interactions between the ribosome surface and nascent polypeptides as the cause of ribosome-induced stability modulations. The study establishes a robust proteomic methodology for analyzing localized stabilities within ribosome-bound nascent polypeptides and sheds light on how the ribosome influences the thermodynamics of protein folding. |
HostingRepository | PRIDE |
AnnounceDate | 2023-08-09 |
AnnouncementXML | Submission_2023-08-08_18:15:35.019.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | RuiyueTan |
SpeciesList | scientific name: Escherichia coli; NCBI TaxID: 562; |
ModificationList | monohydroxylated residue; formylated residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-11-07 09:27:57 | ID requested | |
⏵ 1 | 2023-08-08 18:15:35 | announced | |
2 | 2023-11-14 06:41:28 | announced | 2023-11-14: Updated project metadata. |
Publication List
Keyword List
submitter keyword: Protein stability |
Co-translational protein folding |
Ribosome |
Translation |
Proteomics |
Mass spectrometry (MS) |
Methionine oxidation |
Contact List
SinaGhaemmaghami |
contact affiliation | Ghaemmaghami lab, biology department, University of Rochester |
contact email | sina.ghaemmaghami@rochester.edu |
lab head | |
RuiyueTan |
contact affiliation | University of Rochester |
contact email | rtan12@ur.rochester.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD037995
- Label: PRIDE project
- Name: Folding stabilities of ribosome-bound nascent polypeptides probed by mass spectrometry