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PXD037971-1

PXD037971 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSubcellular Localization Shapes the Fate of RNA Polymerase III
DescriptionRNA Pol III plays a vital role in transcription and as a viral-DNA sensor, but how it is assembled and distributed within cells remain poorly understood. Here, we show that Pol III is assembled with chaperones in the cytoplasm and forms transcription-dependent protein clusters upon transport into the nucleus. The largest subunit (RPC1) depletion through an auxin-inducible degron leads to rapid degradation and disassembly of Pol III complex in the nucleus and cytoplasm, respectively. This generates a pool of partially assembled Pol III intermediates, which can be rapidly mobilized into the nucleus upon the restoration of RPC1. Our study highlights the critical role of subcellular localization in determining Pol III's fate and provide insight into the dynamic regulation of nuclear Pol III levels and the origin of cytoplasmic Pol III complexes involved in mediating viral immunity.
HostingRepositoryiProX
AnnounceDate2022-11-06
AnnouncementXMLSubmission_2023-08-22_19:51:23.562.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterKai Tian
SpeciesList scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-11-07 00:06:38ID requested
12023-08-22 19:51:23announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: turboID, mESC, pol III, subunit degradation, subcellular fraction
Contact List
Xiong Ji
contact affiliationPeking University
contact emailxiongji@pku.edu.cn
lab head
Kai Tian
contact affiliationPeking university
contact emailtiankai_ioz@163.com
dataset submitter
Full Dataset Link List
iProX dataset URI