PXD037954 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Micro-topographies induce epigenetic reprogramming and quiescence in human mesenchymal stem cells |
Description | Biomaterials can control cell and nuclear morphology. Since the shape of the nucleus influences chromatin architecture, gene expression, and cell identity, surface topography can control cell phenotype. This study explores how surface topography influences nuclear morphology, histone modifications, and expression of histone-associated proteins through advanced histone mass spectrometry and microarray analysis. We found that nuclear confinement is associated with loss of both histone acetylation and nucleoli abundance, while pathway analysis revealed a substantial reduction in gene expression associated with chromosome organization. In light of previous observations where we found a decrease in proliferation and metabolism induced by micro-topographies, we connect these findings with a quiescent phenotype in mesenchymal stem cells, as further shown by a reduction of ribosomal proteins and the maintenance of multipotency on micro-topographies after long-term culture conditions. Furthermore, this influence of micro-topographies on nuclear morphology and proliferation was reversible, as shown by a full return of proliferation when re-cultured on a flat surface. Our findings provide novel insights on how biophysical signaling influences nuclear organization and subsequent cellular phenotype. |
HostingRepository | PRIDE |
AnnounceDate | 2022-12-03 |
AnnouncementXML | Submission_2022-12-03_11:36:03.608.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | MaartenDhaenens |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monomethylated residue; acetylated residue; formylated residue |
Instrument | Synapt MS |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-11-04 09:05:17 | ID requested | |
⏵ 1 | 2022-12-03 11:36:04 | announced | |
2 | 2023-11-14 08:14:35 | announced | 2023-11-14: Updated project metadata. |
Publication List
Vermeulen S, Van Puyvelde B, Bengtsson Del Barrio L, Almey R, van der Veer BK, Deforce D, Dhaenens M, de Boer J, Micro-Topographies Induce Epigenetic Reprogramming and Quiescence in Human Mesenchymal Stem Cells. Adv Sci (Weinh), 10(1):e2203880(2022) [pubmed] |
Keyword List
submitter keyword: Nucleus, epigenetics, biomaterials, mesenchymal stem cells, mechanobiology, DDA |
Contact List
MaartenDhaenens |
contact affiliation | ProGenTomics, Laboratory of Pharmaceutical Biotechnology,Ghent University |
contact email | maarten.dhaenens@ugent.be |
lab head | |
MaartenDhaenens |
contact affiliation | Faculity of Pharmaceutical Biotechnology |
contact email | maarten.dhaenens@ugent.be |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD037954
- Label: PRIDE project
- Name: Micro-topographies induce epigenetic reprogramming and quiescence in human mesenchymal stem cells