PXD037933-2

PXD037933 is an original dataset announced via ProteomeXchange.

Title	Proximity mapping of desmosomes reveals a striking maturation-dependent shift in their molecular neighbourhood
Description	Desmosomes are multiprotein adhesion complexes that link intermediate filaments to the plasma membrane, ensuring the mechanical integrity of cells across tissues, but how they participate in the wider signalling network to exert their full function is unclear. To investigate this we performed multiplexed proximity biotinylation (BioID). The combined interactomes of the essential desmosomal proteins desmocollin 2a, plakoglobin and plakophilin 2a (Pkp2a) in Madin-Darby canine kidney epithelial cells were mapped and their differences and commonalities characterised as desmosome matured from Ca2+-dependence to the mature, Ca2+-independent, hyper-adhesive state, which predominates in tissues. Results suggest distinct roles of individual desmosomal proteins in linking to cellular signalling pathways that alter substantially when cells change their adhesion state. Moreover, we find support for our dualistic concept of desmosomes in which the properties of Pkp2a differ from those of the other, more stable proteins. The data provide a requisite resource to unravel the nature of desmosomes.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:42:06.829.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Craig Lawless
SpeciesList	scientific name: Canis familiaris (Dog) (Canis lupus familiaris); NCBI TaxID: 9615;
ModificationList	biotinylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-03 05:00:31	ID requested
1	2024-05-22 23:59:09	announced
⏵ 2	2024-10-22 06:42:14	announced	2024-10-22: Updated project metadata.

10.1016/j.mcpro.2024.100735;

F, ü, lle JB, de Almeida RA, Lawless C, Stockdale L, Yanes B, Lane EB, Garrod DR, Ballestrem C, Proximity Mapping of Desmosomes Reveals a Striking Shift in Their Molecular Neighborhood Associated With Maturation. Mol Cell Proteomics, 23(3):100735(2024) [pubmed]

submitter keyword: Adhesion,Desmosome, BioID

Christoph Ballestrem
contact affiliation	The Wellcome Centre for Cell-Matrix Research, University of Manchester, Manchester, UK
contact email	christoph.ballestrem@manchester.ac.uk
lab head
Craig Lawless
contact affiliation	University of Manchester
contact email	craig.lawless@manchester.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD037933

PRIDE project URI

• PRIDE
  1. PXD037933
     1. Label: PRIDE project
     2. Name: Proximity mapping of desmosomes reveals a striking maturation-dependent shift in their molecular neighbourhood