PXD037907 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | TMTC1 promotes invasiveness of ovarian cancer |
Description | Ovarian cancer is the most lethal gynecological malignancy and is characterized by peritoneal disseminated metastasis. Although O-mannosyltransferase TMTC1 is highly expressed by ovarian cancer, its pathophysiologic role in ovarian cancer remains unclear. Here, immunohistochemistry showed that TMTC1 was overexpressed in ovarian cancer tissues compared with the adjacent non-tumor ovarian tissues and high TMTC1 expression was associated with poor prognosis in patients with ovarian cancer. Silencing TMTC1 reduced ovarian cancer cell viability, migration, and invasion in vitro as well as suppressed peritoneal tumor growth and metastasis in vivo. Moreover, TMTC1 knockdown reduced cell-laminin adhesion, which was associated with the decreased phosphorylation of FAK at pY397. Conversely, TMTC1 overexpression promoted these malignant properties in ovarian cancer cells. Glycoproteomic analysis and Concanavalin A (ConA) pull-down assays showed that integrins β1 and β4 were novel O-mannosylated protein substrates of TMTC1. Furthermore, TMTC1-mediated cell migration and invasion were significantly reversed by siRNA-mediated knockdown of integrin β1 or β4. Collectively, these results suggest that TMTC1-mediated invasive behaviors are primarily through integrins β1 and β4 and that TMTC1 is a potential therapeutic target for ovarian cancer. |
HostingRepository | PRIDE |
AnnounceDate | 2023-10-24 |
AnnouncementXML | Submission_2023-10-24_11:19:15.545.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Hsin-YiWu |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | mannosylated residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-11-03 04:19:25 | ID requested | |
⏵ 1 | 2023-10-24 11:19:16 | announced | |
2 | 2023-11-14 09:07:29 | announced | 2023-11-14: Updated project metadata. |
Publication List
Yeh TC, Lin NY, Chiu CY, Hsu TW, Wu HY, Lin HY, Chen CH, Huang MC, 4. Cancer Gene Ther, 30(8):1134-1143(2023) [pubmed] |
Keyword List
submitter keyword: O-mannosylation, Ovarian cancer,TMTC1 |
Contact List
Min-ChuanHuang |
contact affiliation | Graduate Institute of Anatomy and Cell Biology, College of Medicine, National Taiwan University |
contact email | mchuang@ntu.edu.tw |
lab head | |
Hsin-YiWu |
contact affiliation | National Taiwan University |
contact email | clairewu.wu@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD037907
- Label: PRIDE project
- Name: TMTC1 promotes invasiveness of ovarian cancer