PXD037901-1

PXD037901 is an original dataset announced via ProteomeXchange.

Title	GABA regulates IL-1β production in macrophages
Description	Neurotransmitters have been well-documented to determine immune cell fates; however, whether and how γ-amino butyric acid (GABA) shapes the function of innate immune cells is still obscure. Here, we demonstrated that GABA orchestrates macrophage maturation and inflammation. GABA treatment during macrophage maturation inhibits interleukin (IL)-1β production from inflammatory macrophages. Mechanistically, GABA enhances succinate-FAD-lysine demethylase1 (LSD1) signaling to regulate the histone demethylation of Bcl2l11 and Dusp2, lowering the formation of NLRP3-ASC-Caspase-1 complex. Meanwhile, GABA-succinate axis lowers succinylation of mitochondrial proteins to promote mitochondrial oxidative phosphorylation (OXPHOS). We also found that GABA alleviates the LPS-induced sepsis as well as high-fat diet-induced obesity in mice. Our study proves that GABA is potential in lessening the pro-inflammatory macrophage responses associating with metabolic reprogramming and protein succinylation, thus providing a strategy for treating macrophage-related inflammatory diseases.
HostingRepository	PRIDE
AnnounceDate	2022-11-29
AnnouncementXML	Submission_2022-11-29_09:06:50.360.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	JianFu
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue
Instrument	autoflex

Revision	Datetime	Status	ChangeLog Entry
0	2022-11-03 04:11:59	ID requested
⏵ 1	2022-11-29 09:06:50	announced
2	2023-01-06 08:32:31	announced	2023-01-06: Updated project metadata.

Dataset with its publication pending

submitter keyword: Macrophage, Succinate, Succinylation,FAD, GABA

JianFu
contact affiliation	South China Agricultural University
contact email	arrow_f1997@qq.com
lab head
JianFu
contact affiliation	South China Agricultural University
contact email	arrow_f1997@qq.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD037901
     1. Label: PRIDE project
     2. Name: GABA regulates IL-1β production in macrophages