PXD037859-1

PXD037859 is an original dataset announced via ProteomeXchange.

Title	Synapse integrity and function: dependence on protein synthesis and identification of potential failure points
Description	Synaptic integrity and function depend on myriad proteins - labile molecules with finite lifetimes that need to be continually replaced with freshly synthesized copies. Here we describe experiments designed to expose synaptic (and neuronal) properties and functions that are particularly sensitive to disruptions in protein supply, identify proteins lost early upon such disruptions, and uncover potential, yet currently underappreciated failure points. We report here that acute suppressions of protein synthesis are followed within hours by reductions in spontaneous network activity levels, impaired oxidative phosphorylation and mitochondrial function, reduced tenacity of both excitatory and inhibitory synapses and loss of synapses of both classes. Conversely, gross impairments in presynaptic vesicle recycling are observed only after several days, as is overt cell death. Proteomic analysis identified groups of potentially essential ‘early-lost’ proteins including regulators of synapse stability, proteins related to bioenergetics, fatty acid and lipid metabolism, and, unexpectedly, numerous proteins involved in Alzheimer’s disease pathology and amyloid beta processing.
HostingRepository	PRIDE
AnnounceDate	2022-11-27
AnnouncementXML	Submission_2022-11-27_00:42:23.370.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	TamarZiv
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-31 09:21:58	ID requested
⏵ 1	2022-11-27 00:42:23	announced

Dataset with its publication pending

submitter keyword: SiLAC, protein synthesis,Synaptic integrity

Professor Noam E.Ziv
contact affiliation	Rappaport Faculty of Medicine and Network Biology Research Laboratories Fishbach Building, room 432 Technion City, Haifa 32000 Israel
contact email	noamz@technion.ac.il
lab head
TamarZiv
contact affiliation	Technion
contact email	tamarz@technion.ac.il
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD037859
     1. Label: PRIDE project
     2. Name: Synapse integrity and function: dependence on protein synthesis and identification of potential failure points