PXD037812 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | HUWE1 interaction partners in HCT116 cells (label free) |
Description | Deregulation of RNA Polymerase II (RNAPII) by oncogenic signaling leads to collisions of RNAPII with DNA synthesis machinery (transcription-replication conflicts, TRCs). TRCs can result in DNA damage and underlie genomic instability in tumor cells. Here we provide evidence that elongating RNAPII promotes activation of the ATM kinase at TRCs to drive DNA repair. We show the ATPase Wrnip1 that binds and protects stalled replication forks, associates with RNAPII and limits ATM activation. Wrnip1 binding to elongating RNAPII requires catalytic activity of the ubiquitin ligase Huwe1. Mutation of Huwe1 promotes the transfer of Wrnip1 onto replisome and induces TRCs stimulating ATM activation on RNAPII. This mechanism is evoked early upon replicative stress to induce Wrnip1 translocation and ATM signaling at TRCs. Thus, although primarily considered as genotoxic events, TRCs can provide a mechanism to maintain genome stability under replicative stress. |
HostingRepository | PRIDE |
AnnounceDate | 2023-07-31 |
AnnouncementXML | Submission_2023-07-31_05:24:32.545.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | EliasEinig |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-10-28 07:37:48 | ID requested | |
⏵ 1 | 2023-07-31 05:24:32 | announced | |
2 | 2023-08-25 22:34:48 | announced | 2023-08-26: Updated project metadata. |
3 | 2023-11-14 09:04:55 | announced | 2023-11-14: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Human, WRNIP1, HCT116, HUWE1 |
Contact List
NikitaPopov |
contact affiliation | Medical Oncology and Pulmonology, University Hospital Tübingen, Germany |
contact email | nbpopov@gmail.com |
lab head | |
EliasEinig |
contact affiliation | Medical Oncology and Pulmonology, University Hospital Tübingen, Germany |
contact email | einig.elias@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD037812
- Label: PRIDE project
- Name: HUWE1 interaction partners in HCT116 cells (label free)