PXD037801-2

PXD037801 is an original dataset announced via ProteomeXchange.

Title	Bile Salts Enhance the Susceptibility of the Allergenic Lipid Transfer Protein from Peach, Pru p 3 to in vitro gastrointestinal digestion
Description	Sensitisation to the lipid transfer protein Pru p 3 is associated with severe allergic reactions to peach, the proteins stability being thought to play a role in its allergenicity. Lipid binding increases susceptibility of Pru p 3 to digestion and so the impact of bile salts on the in vitro gastrointestinal digestibility of Pru p 3 was investigated and digestion products mapped by SDS PAGE and mass spectrometry. Bile salts enhanced the digestibility of Prup3 resulting in an ensemble of around 100 peptides spanning the proteins sequence which were linked by disulphide bonds into structures of ~5-6kDa. IgE binding studies with a serum panel from peach allergic subjects showed digestion reduced, but did not abolish, the IgE reactivity of Pru p 3. These data show the importance of including bile salts in in vitro digestion systems and emphasise the need to profile of digestion in a manner that allows identification of immunologically relevant disulphide-linked peptide aggregates.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:09:36.062.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Matthew Daly
SpeciesList	scientific name: Prunus persica; NCBI TaxID: 3760;
ModificationList	phosphorylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Xevo G2-XS QTof

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-28 07:21:17	ID requested
1	2023-10-24 12:29:35	announced
⏵ 2	2023-11-14 09:09:40	announced	2023-11-14: Updated project metadata.
3	2024-10-22 06:09:46	announced	2024-10-22: Updated project metadata.

Wang K, Gali-Moya J, Ruano-Zaragoza M, Cain K, D'Auria G, Daly M, Barran P, Crevel R, Mills ENC, Bile salts enhance the susceptibility of the peach allergenic lipid transfer protein, Pru p 3, to in vitro gastrointestinal proteolysis. Sci Rep, 13(1):15155(2023) [pubmed]

ProteomeXchange project tag: Biology/Disease-Driven Human Proteome Project (B/D-HPP)

submitter keyword: disulphide bonds, immunoactivity, digestibility, bile salts,Pru p 3

Clare Mills
contact affiliation	Manchester Institute of Biotechnology, School of Biological Sciences, Manchester Academic Health Sciences Centre, The University of Manchester, Princess Street, Manchester, M1 7DN, UK
contact email	clare.mills@manchester.ac.uk
lab head
Matthew Daly
contact affiliation	University of Manchester
contact email	matthew.daly-3@postgrad.manchester.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD037801
     1. Label: PRIDE project
     2. Name: Bile Salts Enhance the Susceptibility of the Allergenic Lipid Transfer Protein from Peach, Pru p 3 to in vitro gastrointestinal digestion