PXD037794-1
PXD037794 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteome of senescent human lung fibroblasts and Panc-1 cells |
| Description | Proteomic analysis of proliferating and senescent normal healthy lung fibroblasts (NHLFs) and Panc-1 cells. Senescence was induced using 5 uM Palbociclib for 6 days. Cells were lysed in SDS lysis buffer (2% SDS in 100 mM TEAB), boiled (15 min, 95 °C, 500 rpm) and sonicated in a sonication bath (10 min). DNA was sheared with Benzonase® Nuclease (Merck Millipore) and 2 mM MgCl2 at 37 °C (30 min, 500 rpm). Reversibly oxidized cysteines were reduced with 10 mM TCEP followed by alkylation with 20 mM IAA (each step 30 min, 22 °C, 600 rpm, in the dark). Proteins were acetone precipitated and the protein pellet was resuspended in 100 µL 100 mM TEAB. Proteins were digested overnight with LysC/trypsin (1:50 protease:protein ratio, 37 °C, 600 rpm) Peptides were desalted by solid-phase extraction using Oasis HLB cartridges (10 mg; Waters), resuspended in 3% ACN/0.1% TFA and analysed on an Orbitrap Eclipse Tribrid mass spectrometer in combination with the FAIMS Pro Interface (Thermo Scientific). |
| HostingRepository | MassIVE |
| AnnounceDate | 2022-11-30 |
| AnnouncementXML | Submission_2022-11-30_13:24:43.757.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Eva Griesser |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | Carbamidomethyl; Oxidation; Deamidated; Gln->pyro-Glu |
| Instrument | Orbitrap Eclipse |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2022-10-28 04:46:45 | ID requested | |
| ⏵ 1 | 2022-11-30 13:24:44 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: senescence, NHLF, PANC-1, fibroblasts, lung, palbociclib |
Contact List
| Karim C. El Kasmi | |
|---|---|
| contact affiliation | Boehringer Ingelheim Pharma GmbH & Co. KG, Biberach an der Riss |
| contact email | karim_christian.el_kasmi@boehringer-ingelheim.com |
| lab head | |
| Eva Griesser | |
| contact affiliation | Boehringer Ingelheim Pharma Gmbh & Co. KG |
| contact email | eva.griesser@boehringer-ingelheim.com |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000090607/ |
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