PXD037768-1

PXD037768 is an original dataset announced via ProteomeXchange.

Title	Seb1 proximity dependent biotinylation (PDB) in Schizosaccharomyces pombe
Description	Transcription by RNA polymerase I (RNAPI) represents most of the transcriptional activity in eukaryotic cells and is associated with the production of mature ribosomal RNA (rRNA). As several rRNA maturation steps are coupled to RNAPI transcription, the rate of RNAPI elongation directly influences processing of nascent pre-rRNA, and changes in RNAPI transcription rate can result in alternative rRNA processing pathways in response to growth conditions and stress. However, factors and mechanisms that control RNAPI progression by influencing transcription elongation rate remain poorly understood. Our project is to show that the conserved RNA-binding Seb1 is a pausing-promoting factor for RNA polymerases I to control cotranscriptional RNA processing. Here, we did a proximity dependent biotinylation followed by mass spectrometry (PDB-MS) of the Seb1 protein in order to assess for physical interactions with the RNAPI transcription machinery. A mutant E. coli BirA enzyme is fused to the Seb1 protein. This mutant version of BirA uses biotin to catalyze the formation of biotinoyl-5′-AMP (bioAMP), thereby generating a ‘cloud’ of activated biotin molecules that can react with free primary amines (most often lysine residues) of neighboring proteins. This experiment will support the conclusion that Seb1 is located at the rDNA locus.
HostingRepository	PRIDE
AnnounceDate	2023-05-02
AnnouncementXML	Submission_2023-05-02_08:28:12.345.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	FrançoisBachand
SpeciesList	scientific name: Schizosaccharomyces pombe; NCBI TaxID: 4896;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-27 02:50:54	ID requested
⏵ 1	2023-05-02 08:28:12	announced

Dataset with its publication pending

submitter keyword: Schizosaccharomyces pombe, Seb1, turboID

FrançoisBachand
contact affiliation	Departement of biochemistry and functional genomic, Sherbrooke university, Sherbrooke, Quebec, Canada
contact email	f.bachand@usherbrooke.ca
lab head
FrançoisBachand
contact affiliation	Université de Sherbrooke
contact email	f.bachand@usherbrooke.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/05/PXD037768

PRIDE project URI

• PRIDE
  1. PXD037768
     1. Label: PRIDE project
     2. Name: Seb1 proximity dependent biotinylation (PDB) in Schizosaccharomyces pombe