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PXD037768 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSeb1 proximity dependent biotinylation (PDB) in Schizosaccharomyces pombe
DescriptionTranscription by RNA polymerase I (RNAPI) represents most of the transcriptional activity in eukaryotic cells and is associated with the production of mature ribosomal RNA (rRNA). As several rRNA maturation steps are coupled to RNAPI transcription, the rate of RNAPI elongation directly influences processing of nascent pre-rRNA, and changes in RNAPI transcription rate can result in alternative rRNA processing pathways in response to growth conditions and stress. However, factors and mechanisms that control RNAPI progression by influencing transcription elongation rate remain poorly understood. Our project is to show that the conserved RNA-binding Seb1 is a pausing-promoting factor for RNA polymerases I to control cotranscriptional RNA processing. Here, we did a proximity dependent biotinylation followed by mass spectrometry (PDB-MS) of the Seb1 protein in order to assess for physical interactions with the RNAPI transcription machinery. A mutant E. coli BirA enzyme is fused to the Seb1 protein. This mutant version of BirA uses biotin to catalyze the formation of biotinoyl-5′-AMP (bioAMP), thereby generating a ‘cloud’ of activated biotin molecules that can react with free primary amines (most often lysine residues) of neighboring proteins. This experiment will support the conclusion that Seb1 is located at the rDNA locus.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
SpeciesList scientific name: Schizosaccharomyces pombe; NCBI TaxID: 4896;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-10-27 02:50:54ID requested
12023-05-02 08:28:12announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Schizosaccharomyces pombe, Seb1, turboID
Contact List
contact affiliationDepartement of biochemistry and functional genomic, Sherbrooke university, Sherbrooke, Quebec, Canada
contact emailf.bachand@usherbrooke.ca
lab head
contact affiliationUniversité de Sherbrooke
contact emailf.bachand@usherbrooke.ca
dataset submitter
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Dataset FTP location
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