PXD037758-2

PXD037758 is an original dataset announced via ProteomeXchange.

Title	Essential protein P116 scavenges cholesterol and other indispensable lipids for Mycoplasmas
Description	Mycoplasma pneumoniae, responsible for approximately 30% of community-acquired human pneumonia, needs to extract lipids from the host environment for survival and proliferation. Here, we report a comprehensive structural and functional analysis of the previously uncharacterized protein P116 (MPN_213). Single-particle cryo-electron microscopy of P116 reveals a homodimer presenting a previously unseen fold, forming a huge hydrophobic cavity, which is fully accessible to solvent. Lipidomics analysis shows that P116 specifically acquires lipids such as phosphatidylcholine, sphingomyelin and cholesterol. Structures of different conformational states reveal the mechanism by which lipids are scavenged. This finding immediately suggests a way to control Mycoplasma infection by interfering with lipid uptake.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:58:24.796.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Julian Langer
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-27 02:38:04	ID requested
1	2023-04-19 04:51:19	announced
⏵ 2	2023-11-14 08:58:25	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: mycoplasma,P116, timsTOF

Julian D. Langer
contact affiliation	Proteomics, Max Planck Institute of Biohysics, Frankfurt, Germany Proteomics, Max Planck Institute Brain Research, Frankfurt, Germany
contact email	julian.langer@biophys.mpg.de
lab head
Julian Langer
contact affiliation	MPIs for Biophysics and Brain Research
contact email	julian.langer@biophys.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/04/PXD037758

PRIDE project URI

• PRIDE
  1. PXD037758
     1. Label: PRIDE project
     2. Name: Essential protein P116 scavenges cholesterol and other indispensable lipids for Mycoplasmas