PXD037741-1

PXD037741 is an original dataset announced via ProteomeXchange.

Title	Mitochondrial dysfunction rapidly modulates the abundance and thermal stability of cellular proteins
Description	Cellular functionality relies on a well-balanced, but highly dynamic proteome. Dysfunctionof mitochondrial protein import leads to the cytosolic accumulation of mitochondrial precursor proteins which compromise cellular proteostasis and trigger the mitoprotein-induced stress response. To dissect the effects of mitochondrial dysfunction on the cellular proteome as a whole, we developed pre-post thermal proteome profiling (ppTPP). This multiplexed time-resolved proteome-wide thermal stability profiling approach with isobaric peptide tags in combination with a pulse SILAC labeling elucidated dynamic proteostasis changes in several dimensions: In addition to adaptations in protein abundance, we observed rapid modulations of the thermal stability of individual cellular proteins. Strikingly, different functional groups of proteins showed characteristic response patterns and reacted with group-specific kinetics, allowing the identification of the functional modules that are relevant for mitoprotein-induced stress. Thus, our new ppTPP approach uncovered a complex response network that orchestrates proteome homeostasis in eukaryotic cells by time-controlled adaptations of protein abundance and protein stability.
HostingRepository	PRIDE
AnnounceDate	2023-02-21
AnnouncementXML	Submission_2023-02-21_09:54:55.803.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	FrankStein
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-26 15:46:35	ID requested
⏵ 1	2023-02-21 09:54:56	announced

Dataset with its publication pending

submitter keyword: Mitochondria / Proteasome / Protein folding / Proteostasis / Thermal proteome profiling / Stress response

Johannes M.Herrmann
contact affiliation	Univeristy of Kaiserslautern Cell Biology
contact email	hannes.herrmann@bio.uni-kl.de
lab head
FrankStein
contact affiliation	EMBL
contact email	frank.stein@embl.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD037741
     1. Label: PRIDE project
     2. Name: Mitochondrial dysfunction rapidly modulates the abundance and thermal stability of cellular proteins