PXD037734-2

PXD037734 is an original dataset announced via ProteomeXchange.

Title	A seven-transmembrane methyltransferase catalysing N-terminal histidine methylation of lytic polysaccharide monooxygenases
Description	Lytic polysaccharide monooxygenases (LPMOs) are oxidative enzymes that help break down lignocellulose, making them highly attractive for improving biomass utilization in biotechnological purposes. The catalytically essential N-terminal histidine (His1) of LPMOs is post-translationally modified by methylation in filamentous fungi to protect them from auto-oxidative inactivation, however, the responsible methyltransferase enzyme is unknown. Using mass-spectrometry-based quantitative proteomics in combination with systematic CRISPR/Cas9 knockout screening in Aspergillus nidulans, we identified the N-terminal histidine methyltransferase (NHMT) encoded by the gene AN4663. Targeted proteomics confirmed that NHMT was solely responsible for His1 methylation of LPMOs. NHMT is predicted to encode a unique seven-transmembrane segment anchoring a soluble methyltransferase domain. Co-localization studies showed endoplasmic reticulum residence of NHMT and co-expression in the industrial production yeast Komagataella phaffii with LPMOs resulted in His1 methylation of the LPMOs. This demonstrates the biotechnological potential of recombinant production of proteins and peptides harbouring this unique post-translational modification.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:11:26.717.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tanveer Batth
SpeciesList	scientific name: Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans); NCBI TaxID: 227321; scientific name: Komagataella pastoris (strain GS115 / ATCC 20864) (Yeast) (Pichia pastoris); NCBI TaxID: 644223;
ModificationList	methylated histidine
Instrument	Orbitrap Exploris 480; Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-26 01:25:09	ID requested
1	2023-07-14 07:33:54	announced
⏵ 2	2023-11-14 07:11:27	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: Methyltransferase,LPMO, PTM, LCMS, fungi

Jesper Velgaard Olsen
contact affiliation	Vice Director, Professor Novo Nordisk Foundation Center for Protein Research Proteomics Program University of Copenhagen Denmark
contact email	jesper.olsen@cpr.ku.dk
lab head
Tanveer Batth
contact affiliation	Novo Nordisk Foundation Center for Protein Research, University of Copenhagen
contact email	t.batth@cpr.ku.dk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD037734
     1. Label: PRIDE project
     2. Name: A seven-transmembrane methyltransferase catalysing N-terminal histidine methylation of lytic polysaccharide monooxygenases