PXD037675 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The massive solubility changes of neuronal proteins upon simulated traumatic brain injury |
Description | The molecular consequences of traumatic brain injuries or TBI are poorly understood. Here, we simulated TBI using an innovative laboratory apparatus employing a 5.1 kg dummy impact generating a ≤3,300 g-force acceleration and performed system-wide profiling of neuronal cells using Proteome Integral Solubility Alteration (PISA) assay. Dynamic impact leads to both reduction in neuron viability and massive solubility changes in the proteome. The affected proteins map not only to pathways such as cell adhesion, collagen and laminin structures, as well as response to stress, but also to other dense protein networks, such as immune response, complement and coagulation cascades. An energy absorbing material largely rescues the loss of cell viability and dampens proteome solubility changes. The cellular effects are found to be mainly due to the shockwave rather than the g-force acceleration. This study paves the way to introducing a proteome-based shock-meter as well as identifying TBI protein biomarkers and potential drug targets for prevention and intervention. |
HostingRepository | PRIDE |
AnnounceDate | 2023-09-23 |
AnnouncementXML | Submission_2023-09-23_05:11:53.754.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Amir AtaSaei |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-10-24 01:58:50 | ID requested | |
⏵ 1 | 2023-09-23 05:11:54 | announced | |
2 | 2023-11-14 07:49:31 | announced | 2023-11-14: Updated project metadata. |
Publication List
Keyword List
submitter keyword: PISA, proteomics, neurons, shock, TBI, dynamic impact, stability |
Contact List
Prof. Roman A.Zubarev |
contact affiliation | Division of Physiological Chemistry I, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, 171 65 Stockholm, Sweden |
contact email | Roman.zubarev@ki.se |
lab head | |
Amir AtaSaei |
contact affiliation | Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden |
contact email | amirata.saei.dibavar@ki.se |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/09/PXD037675 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD037675
- Label: PRIDE project
- Name: The massive solubility changes of neuronal proteins upon simulated traumatic brain injury