PXD037661 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Inherited anemia-associated mutations disrupt Codanin1 and CDIN1 mutual direct binding |
| Description | Congenital dyserythropoietic anemia type I (CDA-I) is a rare hereditary disease that causes ineffective erythropoiesis and morphological abnormalities in bone marrow erythroblasts. The congenital abnormalities are manifested by internuclear bridges and Swiss-cheese-like heterochromatin. CDA-I is associated with mutations in two proteins, Codanin1 and CDIN1. Codanin1 is involved in nucleosome assembly and disassembly. CDIN1 is a recently discovered protein with a predicted structure similar to an endonuclease. The critical importance of Codanin1 and CDIN1 for CDA-I progression merited a thorough investigation of the structure-function relations of both proteins. Here, we reveal essential interacting regions between CDIN1 and Codanin1. We described the structural envelopes and the stoichiometry of proteins. Additionally, we quantified CDIN1-Codanin1 binding affinity in the low nanomolar range. Finally, we show how the anemia-associated mutations residing in defined interaction regions disturb the direct interaction of CDIN1 and Codanin1. The presented pivotal study of the structure and function of Codanin1 and CDIN1 is a significant step toward unraveling the CDA-I activation and progression mechanisms at the molecular level. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-09-07 |
| AnnouncementXML | Submission_2026-09-06_16:16:30.353.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Lenka Hernychova |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | LTQ Orbitrap Elite |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2022-10-24 01:06:53 | ID requested | |
| ⏵ 1 | 2026-09-06 16:16:31 | announced | |
Publication List
| Stojaspal M, Brom T, Ne, č, asov, á I, Janovi, č T, Veverka P, Verma N, Uhr, í, k L, Hernychova L, Hofr C, Anemia-associated mutations disrupt the CDIN1-Codanin1 complex in inherited congenital dyserythropoietic anemia I (CDA-I) disease. FEBS J, 293(14):4288-4305(2026) [pubmed] |
| 10.1111/febs.70421; |
Keyword List
| submitter keyword: Rare diseases, CDIN1, LC-MSMS, C15ORF41, Codanin1, congenital dyserythropoietic anemia, HDX-MS |
Contact List
| doc. Mgr. Ctirad Hofr, Ph.D. |
|---|
| contact affiliation | National Centre for Biomolecular Research Faculty of Science, Masaryk University Kamenice 753/5, 625 00 Brno, Czech Republic |
| contact email | hofr@sci.muni.cz |
| lab head | |
| Lenka Hernychova |
|---|
| contact affiliation | Masaryk Memorial Cancer Institutre, Zluty kopec 7, Brno 656 53, Czech Republic |
| contact email | lenka.hernychova@mou.cz |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD037661
- Label: PRIDE project
- Name: Inherited anemia-associated mutations disrupt Codanin1 and CDIN1 mutual direct binding
