PXD037622-3

PXD037622 is an original dataset announced via ProteomeXchange.

Title	Increased lipid transport and de novo synthesis lead to dynamic lipidome remodeling and regulation of lipid-dependent kinases during megakaryopoiesis and proplatelet formation
Description	During megakaryopoiesis and consecutive platelet production, megakaryocytes undergo robust cellular morphological changes which are associated with the reprogramming of signaling pathways. Moreover, it can be assumed that lipid membrane composition and signaling are strongly modified. However, the knowledge of lipid alteration during megakaryopoiesis and which pathways are involved is still lacking. Here, we use a lipid-centric multiomics approach to create a quantitative map of the megakaryocyte lipidome during maturation and proplatelet formation. Our data reveal that megakaryocyte differentiation is driven by an increased fatty acyl import and de novo lipid synthesis, resulting in the modulation towards an anionic membrane phenotype. This phenotype and the upregulation of diacylglycerols highly correlate with the activation of lipid-dependent kinases such as PKC and BTK. Using inhibitors of fatty acid import and phospholipid synthesis proved to block membrane remodeling and kinase activation and directly reduced proplatelet formation. Altogether, this study provides a framework for understanding how membrane lipid remodeling during megakaryocyte differentiation impacts kinase signaling and proplatelet formation while providing a knowledge base to exploit megakaryopoiesis.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:01:12.672.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christopher Gerner
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-20 13:35:25	ID requested
1	2023-08-22 01:06:20	announced
2	2023-11-14 07:08:27	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 06:01:13	announced	2024-10-22: Updated project metadata.

10.1038/S44161-023-00325-8;

submitter keyword: megakaryocytes, lipid-dependent kinases,proteomics, lipidomics, megakaryopoiesis, LC-MS/MS

Christopher Gerner
contact affiliation	University of Vienna, Faculty of Chemistry, Department of Analytical Chemistry
contact email	christopher.gerner@univie.ac.at
lab head
Christopher Gerner
contact affiliation	University of Vienna
contact email	christopher.gerner@univie.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/08/PXD037622

PRIDE project URI

• PRIDE
  1. PXD037622
     1. Label: PRIDE project
     2. Name: Increased lipid transport and de novo synthesis lead to dynamic lipidome remodeling and regulation of lipid-dependent kinases during megakaryopoiesis and proplatelet formation