PXD037617 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | RBPMS mediates smooth muscle specific alternative splicing via oligomerization and modulation of trans factor recruitment |
Description | RBPMS regulate splicing decisions by modulating the transcript-bound proteome. This experiment aims to investigate the proteome assembled on an experimental RNA, TM3 transcript, in human Hela nuclear extract under the various conditions, ± ATP and ± RBPMS. The model RNA is tethered on amylose bead via a protein, MPB-MS2. After pull-down, the protein-RNA complexes are eluted from the beads with maltose. The sample series "N" and "NA" are two negative control conditions. "B" and "R" are sample series of two experimental conditions. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_09:06:45.154.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Yi Yang |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-10-20 13:28:21 | ID requested | |
1 | 2023-10-24 10:47:09 | announced | |
⏵ 2 | 2023-11-14 09:06:47 | announced | 2023-11-14: Updated project metadata. |
Publication List
Yang Y, Lee GC, Nakagaki-Silva E, Huang Y, Peacey M, Partridge R, Gooding C, Smith CWJ, Cell-type specific regulator RBPMS switches alternative splicing via higher-order oligomerization and heterotypic interactions with other splicing regulators. Nucleic Acids Res, 51(18):9961-9982(2023) [pubmed] |
Keyword List
submitter keyword: LC MS/MS, RNA, alternative splicing factor,human |
Contact List
Chris WJ Smith |
contact affiliation | Department of Biochemistry University of Cambridge |
contact email | cwjs1@cam.ac.uk |
lab head | |
Yi Yang |
contact affiliation | Department of Biochemistry, University of Cambridge |
contact email | yy462@cam.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD037617
- Label: PRIDE project
- Name: RBPMS mediates smooth muscle specific alternative splicing via oligomerization and modulation of trans factor recruitment