PXD037611-1

PXD037611 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Ammonium Sulfate-based Prefractionation Improved Carbonylated Proteome Coverage in Arabidopsis thaliana Leaf Extract
Description	The hypothesis that ammonium sulfate-based protein precipitation can help prefractionate the total protein extract into fractions of reduced complexity and improve the detection of low-abundance proteins was investigated. This dataset resulted from the experiments that we performed to improve the detection of the carbonylated proteins in leaf extract. A total of 122 carbonylated proteins were identified, of which 77 were found in the ammonium sulfate fractions only. This prefractionation method allowed to identify 63% more carbonylated proteins compared to the number of carbonylated proteins identified from the total crude extract without prefractionation. Our results thus showed that a simple offline prefractionation of the proteome by ammonium sulfate prior to liquid chromatography-tandem mass spectrometry significantly improved the detection of carbonylated proteins in Arabidopsis leaf extract.
HostingRepository	MassIVE
AnnounceDate	2023-02-19
AnnouncementXML	Submission_2023-02-19_10:44:13.059.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Tagnon Missihoun
SpeciesList	scientific name: Arabidopsis thaliana; common name: thale cress; NCBI TaxID: 3702;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-20 11:25:25	ID requested
⏵ 1	2023-02-19 10:44:13	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: Aldehyde reactive probe, peptide level enrichment, protein carbonylation, proteome offline prefractionation

submitter keyword: Aldehyde reactive probe, peptide level enrichment, protein carbonylation, proteome offline prefractionation

Contact List

Tagnon Missihoun
contact affiliation	University of Quebec Trois-Rivieres
contact email	Tagnon.Missihoun@uqtr.ca
lab head
Tagnon Missihoun
contact affiliation	University of Quebec Trois-Rivieres
contact email	Tagnon.Missihoun@uqtr.ca
dataset submitter

Full Dataset Link List

MassIVE dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000090559/

MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000090559/