PXD037596 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | RhoA GEFs (Lfc subcellular localization and activity is controlled by αv-class integrin) |
Description | Fibronectin (FN)-binding integrins control a variety of cellular responses through Rho GTPases. The FN-binding integrins, αvβ3 and α5β1, are known to induce different effects on cell morphology and motility. Here we report that FN-bound αvβ3 integrin, but not FN-bound α5β1 integrin, triggers the dissociation of the RhoA GEF Lfc (GEF-H1 in humans) from microtubules (MT), leading to the activation of RhoA, formation of stress fibres and maturation of focal adhesions (FAs). Conversely, loss of Lfc expression decreases RhoA activity, stress fibre formation and FA size, suggesting that Lfc is the major GEF downstream of FN-bound αvβ3 that controls RhoA activity. Mechanistically, FN-engaged αvβ3 integrin activates a kinase cascade involving MARK2/3, which in turn leads to phosphorylation of several phospho-sites on Lfc. In particular, S151 was identified as the main site involved in the regulation of Lfc localization and activity. Our findings indicate that activation of Lfc/RhoA is orchestrated in FN-adherent cells in an integrin-specific manner. |
HostingRepository | PRIDE |
AnnounceDate | 2023-04-17 |
AnnouncementXML | Submission_2023-04-17_11:49:43.946.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | GEORGINACOLO |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | acetylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-10-20 02:18:32 | ID requested | |
⏵ 1 | 2023-04-17 11:49:44 | announced | |
2 | 2023-11-14 08:58:18 | announced | 2023-11-14: Updated project metadata. |
Publication List
Keyword List
submitter keyword: RhoA,GEF, mouse |
Contact List
Georgina PamelaColó |
contact affiliation | Cancer Biology lab, Instituto de Investigaciones Bioquímicas de Bahía Blanca (CONICET), Departamento de Biología, Bioquímica y Farmacia, Universidad Nacional del Sur, Bahía Blanca, Argentina |
contact email | gcolo@inibibb-conicet.gob.ar |
lab head | |
GEORGINACOLO |
contact affiliation | CONICET |
contact email | gcolo@inibibb-conicet.gob.ar |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD037596
- Label: PRIDE project
- Name: RhoA GEFs (Lfc subcellular localization and activity is controlled by αv-class integrin)