PXD037528-1

PXD037528 is an original dataset announced via ProteomeXchange.

Title	SND1 binds SARS-CoV-2 negative-sense RNA and promotes viral RNA synthesis through NSP9
Description	Regulation of viral RNA biogenesis is fundamental to productive SARS-CoV-2 infection. To characterize host RNA-binding proteins (RBPs) involved in this process, we biochemically identified proteins bound to genomic and subgenomic SARS-CoV-2 RNAs. We find that the host protein SND1 binds the 5ʹ end of negative-sense viral RNA and is required for SARS-CoV-2 RNA synthesis. SND1-depleted cells form smaller replication organelles and display diminished virus growth kinetics. We discover that NSP9, a viral RBP and direct SND1 interaction partner, is covalently linked to the 5ʹ ends of positive and negative-sense RNAs produced during infection. These linkages occur at replication-transcription initiation sites, consistent with NSP9 priming viral RNA synthesis. Mechanistically, SND1 remodels NSP9 occupancy and alters the covalent linkage of NSP9 to initiating nucleotides in viral RNA. Our findings implicate NSP9 in the initiation of SARS-CoV-2 RNA synthesis and unravel an unsuspected role of a cellular protein in orchestrating viral RNA production.
HostingRepository	PRIDE
AnnounceDate	2023-10-05
AnnouncementXML	Submission_2023-10-04_23:24:09.965.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	MathiasMunschauer
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-18 08:02:50	ID requested
⏵ 1	2023-10-04 23:24:10	announced
2	2023-11-14 07:49:31	announced	2023-11-14: Updated project metadata.

ProteomeXchange project tag: Sars-cov-2, Covid-19

submitter keyword: SARS-CoV-2, RNA-binding proteins, RNA interactome

MathiasMunschauer
contact affiliation	Helmholtz Institute for RNA-based Infection Research, Helmholtz-Center for Infection Research, Würzburg, Germany Faculty of Medicine, University of Würzburg, Würzburg, Germany
contact email	mathias.munschauer@helmholtz-hiri.de
lab head
MathiasMunschauer
contact affiliation	Helmholtz Institute for RNA-based Infection Research
contact email	mathias.munschauer@helmholtz-hiri.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD037528

PRIDE project URI

• PRIDE
  1. PXD037528
     1. Label: PRIDE project
     2. Name: SND1 binds SARS-CoV-2 negative-sense RNA and promotes viral RNA synthesis through NSP9