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PXD037528-1

PXD037528 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSND1 binds SARS-CoV-2 negative-sense RNA and promotes viral RNA synthesis through NSP9
DescriptionRegulation of viral RNA biogenesis is fundamental to productive SARS-CoV-2 infection. To characterize host RNA-binding proteins (RBPs) involved in this process, we biochemically identified proteins bound to genomic and subgenomic SARS-CoV-2 RNAs. We find that the host protein SND1 binds the 5ʹ end of negative-sense viral RNA and is required for SARS-CoV-2 RNA synthesis. SND1-depleted cells form smaller replication organelles and display diminished virus growth kinetics. We discover that NSP9, a viral RBP and direct SND1 interaction partner, is covalently linked to the 5ʹ ends of positive and negative-sense RNAs produced during infection. These linkages occur at replication-transcription initiation sites, consistent with NSP9 priming viral RNA synthesis. Mechanistically, SND1 remodels NSP9 occupancy and alters the covalent linkage of NSP9 to initiating nucleotides in viral RNA. Our findings implicate NSP9 in the initiation of SARS-CoV-2 RNA synthesis and unravel an unsuspected role of a cellular protein in orchestrating viral RNA production.
HostingRepositoryPRIDE
AnnounceDate2023-10-05
AnnouncementXMLSubmission_2023-10-04_23:24:09.965.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMathiasMunschauer
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-10-18 08:02:50ID requested
12023-10-04 23:24:10announced
22023-11-14 07:49:31announced2023-11-14: Updated project metadata.
Publication List
Keyword List
ProteomeXchange project tag: Sars-cov-2, Covid-19
submitter keyword: SARS-CoV-2, RNA-binding proteins, RNA interactome
Contact List
MathiasMunschauer
contact affiliationHelmholtz Institute for RNA-based Infection Research, Helmholtz-Center for Infection Research, Würzburg, Germany Faculty of Medicine, University of Würzburg, Würzburg, Germany
contact emailmathias.munschauer@helmholtz-hiri.de
lab head
MathiasMunschauer
contact affiliationHelmholtz Institute for RNA-based Infection Research
contact emailmathias.munschauer@helmholtz-hiri.de
dataset submitter
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Dataset FTP location
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