PXD037517-1

PXD037517 is an original dataset announced via ProteomeXchange.

Title	Proteomic analyses reveal the non-coding small RNA Qrr5 influences autoaggregation and growth competition in Vibrio parahaemolyticus
Description	Vibrio parahaemolyticus, a sea-born bacterial pathogen, is a primary inducement of food-borne gastroenteritis. Most researches have shown that non-coding small RNA plays a vital role in the regulation of multiple biological processes in pathogenic bacteria, especially autoaggregation and growth competition. However, the inherent mechanisms have yet to be fully understood. As important regulators in Vibrios, the involvement of Qrr sRNAs in V. parahaemolyticus is largely unknown. Here, we carried out the Qrr5 deletion mutant and utilized a proteomic method to describe globally proteomic alterations in response to Qrr5 deletion. 297 significantly expressed proteins were determined between the Qrr5 deletion mutant and wild-type strain, among which 137 proteins were upregulated and 160 proteins were downregulated. Upregulated proteins were principally participated in membrane transporters and signal transcription, while downregulated proteins were participated in the two-component system and transcription factor binding. Notably, transcriptional regulator LysR, outer membrane protein OmpA, and conjugal transfer protein TraA-related proteins were upregulated, causing the promotion of autoaggregation ability and growth competition ability against E. coli. This study provides insights into the regulatory network of sRNA in this bacterium, which will facilitate further explorations of important biological processes in pathogenic bacteria.
HostingRepository	iProX
AnnounceDate	2022-10-18
AnnouncementXML	Submission_2023-12-06_19:10:20.257.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Fei Zha
SpeciesList	scientific name: Vibrio parahaemolyticus RIMD 2210633; NCBI TaxID: 223926;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive; Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-17 22:42:12	ID requested
⏵ 1	2023-12-06 19:10:21	announced

Zha F, Pang R, Huang S, Zhang J, Wang J, Chen M, Xue L, Ye Q, Wu S, Yang M, Gu Q, Ding Y, Wu Q, Zhang H, Proteomic analysis reveals the non-coding small RNA Qrr5 influences autoaggregation and growth competition in Vibrio parahaemolyticus. J Proteomics, 279():104866(2023) [pubmed]

submitter keyword: Non-coding small RNA, Qrr5, proteome, autoaggregation, growth competition, Vibrio parahaemolyticus

Qingping Wu
contact affiliation	Guangdong Institute of Microbiology
contact email	wuqp@gdim.cn
lab head
Fei Zha
contact affiliation	Guangdong institute of microbiology
contact email	xiaodylanzha@qq.com
dataset submitter

iProX dataset URI