PXD037509-1

PXD037509 is an original dataset announced via ProteomeXchange.

Title	Complete integration of carbene-transfer chemistry into biosynthesis
Description	Biosynthesis is an environmentally friendly and renewable way to synthesize a broad range of natural and some new-to-nature products; however, it lacks many of the reactions and flexibility of synthetic chemistry, an example being carbene mediated reactions. While it was recently shown that these reactions can be imported into the cell, carbene donors and unnatural cofactors needed to be added exogenously to the cells, precluding cost-effective scale-up of the reaction. Here we identified a biosynthetic gene cluster that when expressed in Streptomyces albus will produce the diazo compound azaserine, which we also demonstrated can serve as a carbene donor across a carbon-carbon double bond in another intracellularly produced molecule, styrene, thereby producing a cyclopropanated product. The reaction was catalyzed with P450 mutants harboring a native cofactor with excellent diastereoselectivity and moderate yield. This work establishes a platform for introducing carbene mediated reactions into microbes for bio-manufacture and contributes to sustainable green chemistry.
HostingRepository	PRIDE
AnnounceDate	2023-05-03
AnnouncementXML	Submission_2023-05-03_09:55:58.277.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	ChristopherPetzold
SpeciesList	scientific name: Streptomyces albus J1074; NCBI TaxID: 457425;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-17 01:54:26	ID requested
⏵ 1	2023-05-03 09:55:58	announced
2	2023-11-14 08:58:18	announced	2023-11-14: Updated project metadata.

submitter keyword: streptomyces albus, P450,Biosynthesis, Carbene meidated reaction

ChristopherPetzold
contact affiliation	Staff Scientist Biological Systems & Engineering Division Lawrence Berkeley National Laboratory Berkeley CA 94720
contact email	cjpetzold@lbl.gov
lab head
ChristopherPetzold
contact affiliation	Lawrence Berkeley National Laboratory
contact email	cjpetzold@lbl.gov
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/05/PXD037509

PRIDE project URI

• PRIDE
  1. PXD037509
     1. Label: PRIDE project
     2. Name: Complete integration of carbene-transfer chemistry into biosynthesis