PXD037493-2

PXD037493 is an original dataset announced via ProteomeXchange.

Title	Confirmation of the binding site of spermine to ATP13A2 protein
Description	To better understand human ATP13A2-mediated polyamine transport, we used single-particle cryo-electron microscopy to solve high-resolution structures of human ATP13A2. Interestingly, we found polyamine binding at multiple sites distributed along the transmembrane regions in ATP13A2 protein, which has not been reported in previous studies. Therefore, we used cross-linking mass spectrometry (CX-MS) to confirm potential binding sites.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:46:39.724.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	jianqiang Mu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-17 01:31:40	ID requested
1	2023-05-10 13:35:38	announced
⏵ 2	2023-11-14 08:46:44	announced	2023-11-14: Updated project metadata.

Mu J, Xue C, Fu L, Yu Z, Nie M, Wu M, Chen X, Liu K, Bu R, Huang Y, Yang B, Han J, Jiang Q, Chan KC, Zhou R, Li H, Huang A, Wang Y, Liu Z, Conformational cycle of human polyamine transporter ATP13A2. Nat Commun, 14(1):1978(2023) [pubmed]

submitter keyword: ATP13A2, CX-MS, SPM

Zhongmin LIU
contact affiliation	Department of Biology, School of Life Sciences, Southern University of Science and Technology, Shenzhen 518055, Guangdong, China
contact email	liuzm@sustech.edu.cn
lab head
jianqiang Mu
contact affiliation	Southern University of Science and Technology,
contact email	jianqiangmshz@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/05/PXD037493

PRIDE project URI

• PRIDE
  1. PXD037493
     1. Label: PRIDE project
     2. Name: Confirmation of the binding site of spermine to ATP13A2 protein