PXD037490-1

PXD037490 is an original dataset announced via ProteomeXchange.

Title	Synaptic vesicle proteins and ATG9A self-organize in distinct vesicle phases within synapsin condensates
Description	Ectopic expression in fibroblasts of synapsin 1 and synaptophysin is sufficient to generate condensates of vesicles highly reminiscent of synaptic vesicle (SV) clusters and with liquid-like properties. We show that unlike synaptophysin, other major integral SV membrane proteins fail to form condensates with synapsin, but coassemble into the clusters formed by synaptophysin and synapsin in this ectopic expression system. Another vesicle membrane protein, ATG9A, undergoes activity-dependent exo-endocytosis at synapses, raising questions about the relation of ATG9A traffic to the traffic of SVs. We have found that both in fibroblasts and in nerve terminals ATG9A does not coassemble into synaptophysin-positive vesicle condensates but localizes on a distinct class of vesicles that also assembles with synapsin but into a distinct phase. Our findings suggest that ATG9A undergoes differential sorting relative to SV proteins and also point to a dual role of synapsin in controlling clustering at synapses of SVs and ATG9A vesicles
HostingRepository	PRIDE
AnnounceDate	2022-12-24
AnnouncementXML	Submission_2022-12-23_19:44:37.086.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zon WengLai
SpeciesList	scientific name: Cercopithecus aethiops (Green monkey) (Grivet); NCBI TaxID: 9534;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-17 01:28:18	ID requested
⏵ 1	2022-12-23 19:44:37	announced

Dataset with its publication pending

submitter keyword: synaptophysin, synapsin, ATG9A, sypnatic vesicle

Pietro DeCamilli
contact affiliation	Yale University School of Medicine
contact email	pietro.decamilli@yale.edu
lab head
Zon WengLai
contact affiliation	Harvard T. H. Chan School of Public Health
contact email	zlai@hsph.harvard.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/12/PXD037490

PRIDE project URI

• PRIDE
  1. PXD037490
     1. Label: PRIDE project
     2. Name: Synaptic vesicle proteins and ATG9A self-organize in distinct vesicle phases within synapsin condensates