PXD037488-2

PXD037488 is an original dataset announced via ProteomeXchange.

Title	Multigenerational effect of heat stress on the Drosophila melanogaster sperm proteome
Description	The notion that genes are the sole units of heredity and that a barrier exists between soma and germline has been a major hurdle in elucidating the heritability of traits that were observed to follow a non-Mendelian inheritance pattern. It was only after the conception of “epigenetics” by C. H. Waddington that the effect of parental environment on subsequent generations via non-DNA sequence-based mechanisms, such as DNA methylation, chromatin modifications, non-coding RNAs and proteins, could be established in various organisms, now referred to as multigenerational epigenetic inheritance. Despite the growing body of evidence, the male gamete-derived epigenetic factors that mediate the transmission of such phenotypes are seldom explored, particularly in the model organism Drosophila melanogaster. Using the heat stress-induced multigenerational epigenetic inheritance paradigm in a widely used position-effect variegation line of Drosophila, named white-mottled, we have dissected the effect of heat stress on the sperm proteome in the current study. We demonstrate that multiple successive generations of heat stress at the early embryonic stage results in a significant downregulation of proteins associated with translation, chromatin organization, microtubule-based processes, and generation of metabolites and energy in the Drosophila sperms. Based on our findings, we propose chromatin-based epigenetic mechanisms, a well-established mechanism for environmentally induced multigenerational effects, as a plausible way of transmitting heat stress memory via the male germ line in subsequent generations. Moreover, we demonstrate the effect of multiple generations of heat stress on the reproductive fitness of Drosophila, shedding light on the adaptive or maladaptive potential of heat stress-induced multigenerational phenotypes.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:38:52.470.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shagufta Khan
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-17 01:27:45	ID requested
1	2024-05-10 06:22:18	announced
⏵ 2	2024-10-22 06:38:52	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: white- mottled, multigenerational epigenetic inheritance,Drosophila melanogaster, sperm proteome, heat stress

Rakesh Kumar Mishra
contact affiliation	CSIR-Centre for Cellular and Molceular Biology, Hyderabad, India TIGS-Tata Institute for Genetics and Society ,Bengaluru, India
contact email	mishra@ccmb.res.in
lab head
Shagufta Khan
contact affiliation	CSIR-Centre for Cellular and Molecular Biology
contact email	shagufta@csirccmb.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD037488

PRIDE project URI

• PRIDE
  1. PXD037488
     1. Label: PRIDE project
     2. Name: Multigenerational effect of heat stress on the Drosophila melanogaster sperm proteome