PXD037436 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | NLRP6 controls PTEN stability by promoting autophagic degradation of p85α to drive tumorigenesis |
Description | To identify NLRP6-associated proteins in living cells, we applied an APEX2-based labelling method combined with mass spectrometry. In brief, APEX2 was genetically fused to NLRP6. In the presence of hydrogen peroxide, APEX2 catalysed biotin-phenol to become a biotin-phenol radical that covalently bound to NLRP6 neighbouring proteins (<20 nm). The biotinylated proteins were enriched and collected by streptavidin beads. One-dimensional SDS–PAGE followed by liquid chromatography-tandem mass spectrometry (GeLC–MS/MS) was performed to identify the proteins. To detect ubiquitinated p85α, a Flag-tagged p85α plasmid was transfected into cells. The cell lysates were immunoprecipitated with anti-Flag and separated on a 6% SDS–PAGE gel. After in-gel digestion, the resulting peptides were extracted for GeLC–MS/MS analysis. |
HostingRepository | PRIDE |
AnnounceDate | 2023-07-02 |
AnnouncementXML | Submission_2023-07-01_22:38:26.651.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | BowenLi |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-10-14 07:14:50 | ID requested | |
⏵ 1 | 2023-07-01 22:38:27 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: NLRP6,cell |
Contact List
Juncui |
contact affiliation | Sun Yat-sen University |
contact email | cuij5@mail.sysu.edu.cn |
lab head | |
BowenLi |
contact affiliation | Third Affiliated Hospital of Soochow University |
contact email | libowen2012@163.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/07/PXD037436 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD037436
- Label: PRIDE project
- Name: NLRP6 controls PTEN stability by promoting autophagic degradation of p85α to drive tumorigenesis