PXD037423-1

PXD037423 is an original dataset announced via ProteomeXchange.

Title	Characterization of Plasma Membrane Proteins in Stylosanthes Leaves and Roots Using Simplified Enrichment Method
Description	Plant plasma membranes (PMs) play an important role in maintaining the stability of the intracellular environment and exchanging information with the external environment. Therefore, deciphering dynamics of PM proteome provides crucial information for elucidating cellular regulation in response to diverse stimuli. In the study, we developed a simplified method for enriching PM proteins in leaf and root tissues of a tropical forage Stylosanthes by combining differential centrifugation and Brij-58 treatment. Both immunoblot analysis and mass spectrometry demonstrated that the representation and abundance of PM proteins were increased in the enrichment fraction, and the contamination of other organellar proteins was decreased. A total of 425 and 387 proteins were predicted to be PM proteins in leaves and roots respectively. Functional analysis classified these PM proteins into six major categories (transporter, enzyme, receptor, membrane structure protein, vesicular trafficking and chaperone), and orthologs of many PM proteins regulating the responses to abiotic and biotic stresses have been detected. In addition, the PM localization of a newly identified receptor-like kinase, SgRKL1, has been verified. Together, these results show that the simplified PM enrichment method can be successfully applied to different plant tissue types and to study the dynamics of PM proteome of Stylosanthes in response to multiple stresses.
HostingRepository	PRIDE
AnnounceDate	2022-12-14
AnnouncementXML	Submission_2022-12-14_05:02:51.754.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	丽云杨
SpeciesList	scientific name: Stylosanthes guianensis; NCBI TaxID: 62615;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-14 05:54:21	ID requested
⏵ 1	2022-12-14 05:02:52	announced
2	2023-11-14 08:59:11	announced	2023-11-14: Updated project metadata.

submitter keyword: proteomics,plasma membrane, Brij-58 treatment, protein enrichment, Stylosanthes

yang liyun
contact affiliation	Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresource, College of Tropical Crops, Hainan University, Haikou, China
contact email	1058200235@qq.com
lab head
丽云杨
contact affiliation	海南大学
contact email	1058200235@qq.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD037423
     1. Label: PRIDE project
     2. Name: Characterization of Plasma Membrane Proteins in Stylosanthes Leaves and Roots Using Simplified Enrichment Method