PXD037401 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | LncRNA LETS1 promotes TGF--induced EMT and cancer progression |
Description | Transforming growth factor- (TGF-) signaling is a critical driver of epithelial–mesenchymal transition (EMT) and cancer progression. However, the regulatory roles of long non-coding RNAs (lncRNAs) in TGF--induced EMT and cancer progression are not well understood. Here, we identified an unannotated nuclear lncRNA LETS1 (LncRNA Enforcing TGF- Signaling 1) as a novel TGF-/SMAD target gene. Loss of LETS1 attenuates TGF--induced EMT, migration and extravasation in breast and lung cancer cells. LETS1 potentiates TGF-/SMAD signaling by stabilizing cell surface TGF- type I receptor (TRI) and thereby forms a positive feedback loop. Mechanistically, LETS1 inhibits TRI polyubiquitination by inducing the orphan nuclear receptor 4A1 (NR4A1) expression, a critical determinant of a destruction complex for inhibitory SMAD7. An unbiased interactome analysis identified the Nuclear Factor of Activated T Cells (NFAT5) as a protein partner of LETS1 to mediate activation of NR4A1 promoter. Overall, our findings characterize LETS1 as an EMT-promoting lncRNA and elucidate the mechanism by which nuclear LETS1 potentiates TGF- receptor signaling. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_06:50:27.837.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Román González-Prieto |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-10-13 15:31:05 | ID requested | |
1 | 2023-07-31 09:29:27 | announced | |
⏵ 2 | 2023-11-14 06:50:28 | announced | 2023-11-14: Updated project metadata. |
3 | 2024-10-22 05:59:21 | announced | 2024-10-22: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: lncRNA |
TFG-beta |
Cancer |
EMT |
Contact List
Román González-Prieto |
contact affiliation | Genome Proteomics Laboratory, CABIMER-US, Seville, Spain |
contact email | roman.gonzalez@cabimer.es |
lab head | |
Román González-Prieto |
contact affiliation | Andalusian Center for Regenerative Medicine and Cell Biology, CABIMER, University of Seville |
contact email | roman.gonzalez@cabimer.es |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD037401
- Label: PRIDE project
- Name: LncRNA LETS1 promotes TGF--induced EMT and cancer progression