PXD037398 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Mutant p53, mutant KRAS and hyperactive CMYC downstream proteomic programs in lung, colon and pancreatic cancer cell lines |
Description | Human cancer cell lines indicated in the file names, with stably overexpressed Cas9 nuclease, were transfected with (C) control sgRNA or (P) TP53-specific sgRNA, (K) KRAS-specific sgRNA or (M) CMYC-specific sgRNA. Each experiment was done by transfection of sgRNA pair - control (C) or causing a NHEJ-mediated knock-out of the target genes (P, K or M). Samples for proteomics were collected 48h post sgRNA transfection without selection, in three biological replicates each (indicated with numbers 1-3). In cell lines with three activated oncogenes, three separate oncogene-targeting transfections were carried out. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-17 |
AnnouncementXML | Submission_2024-10-17_07:57:49.501.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jacek Wisniewski |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-10-13 15:19:27 | ID requested | |
⏵ 1 | 2024-10-17 07:57:50 | announced | |
Publication List
10.1038/s41419-024-06965-3; |
Grze, ś M, Jaiswar A, Grochowski M, Wojty, ś W, Ka, ź, mierczak W, Olesi, ń, ski T, Lenarcik M, Nowak-Niezgoda M, Ko, ł, os M, Canarutto G, Piazza S, Wi, ś, niewski JR, Walerych D, A common druggable signature of oncogenic c-Myc, mutant KRAS and mutant p53 reveals functional redundancy and competition among oncogenes in cancer. Cell Death Dis, 15(8):638(2024) [pubmed] |
Keyword List
submitter keyword: K-ras, cell lines,Cancer |
Contact List
Jacek R Wisniewski |
contact affiliation | Max-Planck-Institute of Biochemistry |
contact email | jwisniew@biochem.mpg.de |
lab head | |
Jacek Wisniewski |
contact affiliation | Proteomics |
contact email | jwisniew@biochem.mpg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/10/PXD037398 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD037398
- Label: PRIDE project
- Name: Mutant p53, mutant KRAS and hyperactive CMYC downstream proteomic programs in lung, colon and pancreatic cancer cell lines