PXD037364-1
PXD037364 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Breaking the mucin barrier: a new affinity chromatography-mass spectrometry approach to unveil potential cell markers and pathways altered in Pseudomyxoma peritonei |
Description | Pseudomyxoma peritonei (PMP) is a rare, malignant tumour with unknown underlying molecular mechanisms characterised by a progressive accumulation of mucin and secreting tumour cells within the abdomen and pelvis. Cytoreductive surgery (CRS) combined with hyperthermic intraperitoneal chemotherapy (HIPEC) is the only therapeutic option currently available for its treatment and recurrence with a fatal outcome is common despite its use. The lack of information available about the molecular mechanisms underlying this condition is mainly due to the physicochemical characteristics of mucin, whose main purpose is to protect epithelial cells, which makes it inaccessible to a proteomic analysis. In this paper we describe the first protocol available to break this barrier and offer a chance to isolate proteins from soft and hard mucinous tumour tissues. This approach is based on the depletion of glycoproteins, immunoglobins, and albumins by affinity liquid chromatography, which makes the mucin available for label free quantification by mass spectrometry. As in the case of non mucinous tissues, our protocol yields a mix of total proteins and has allowed us to establish the first protein profile in mucinous tumours. In addition, our protein-protein interaction networks and pathway enrichment analyses revealed MUC13 and TFF1 as potential therapeutic targets in this disease. |
HostingRepository | PRIDE |
AnnounceDate | 2024-06-22 |
AnnouncementXML | Submission_2024-06-22_09:05:16.532.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Luz Valero |
SpeciesList | scientific name: Trypanosoma cruzi cruzi; NCBI TaxID: 85057; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | TripleTOF 6600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2022-10-13 06:54:31 | ID requested | |
⏵ 1 | 2024-06-22 09:05:17 | announced |
Publication List
10.1186/s12575-024-00239-0; |
Romero-Ruiz A, Granados-Rodr, í, guez M, Bura FI, Valenzuela-Molina F, Rufi, á, n-And, ú, jar B, Mart, í, nez-L, ó, pez A, Rodr, í, guez-Ortiz L, Ortega-Salas R, Torres-Mart, í, nez M, Moreno-Serrano A, Casta, ñ, o J, Mich, á, n C, Alhama J, V, á, zquez-Borrego MC, Arjona-S, á, nchez Á, Breaking the Mucin Barrier: A New Affinity Chromatography-Mass Spectrometry Approach to Unveil Potential Cell Markers and Pathways Altered in Pseudomyxoma Peritonei. Biol Proced Online, 26(1):13(2024) [pubmed] |
Keyword List
submitter keyword: SWATH, Differential expresion,Pseudomyxoma peritonei, Mucin |
Contact List
Antonio Romero-Ruiz | |
---|---|
contact affiliation | GE09 research in peritoneal and retroperitoneal oncological surgery, Maimonides Biomedical Research Institute of Córdoba (IMIBIC, Instituto Maimónides de Investigación Biomédica de Córdoba), Spain. |
contact email | antonioromeroruiz@gmail.com |
lab head | |
Luz Valero | |
contact affiliation | Universitat de Valencia |
contact email | mluz.valero@uv.es |
dataset submitter |
Full Dataset Link List
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/06/PXD037364 |
PRIDE project URI |
Repository Record List
[ + ]