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PXD037350-1

PXD037350 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Multi-Comparative Thermal Proteome Profiling Uncovers New O-GlcNAc Proteins in System-Wide
Description	Among diverse protein post-translational modifications (PTMs), O-GlcNAcylation, a simple but essential monosaccharide modification, plays crucial roles in cellular processes and is closely related to various diseases. Despite its ubiquity in cells, the properties of low stoichiometry and reversibility are hard nuts to crack in system-wide research of O-GlcNAc. Herein, we developed a novel method employing multi-comparative thermal proteome profiling for OGT substrates discovery. Melting curves of proteins under different treatments were profiled and compared with high reproducibility and consistency. Conse-quently, proteins with significant shifted stabilities caused by OGT and UDP-GlcNAc were screened out, from which new O-GlcNAcylated proteins were uncovered.
HostingRepository	iProX
AnnounceDate	2022-10-13
AnnouncementXML	Submission_2022-12-06_18:21:31.532.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Guoli Wang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-12 22:51:46	ID requested
⏵ 1	2022-12-06 18:21:31	announced

Publication List

Dataset with its publication pending

Keyword List

submitter keyword: O-GlcNAcyaltion

Contact List

Haojie Lu
contact affiliation	Fudan University
contact email	luhaojie@fudan.edu.cn
lab head
Guoli Wang
contact affiliation	Fudan University
contact email	19111510006@fudan.edu.cn
dataset submitter

Full Dataset Link List

iProX dataset URI