PXD037346-1

PXD037346 is an original dataset announced via ProteomeXchange.

Title	A proteomic analysis shows stimulation of light reactions and inhibition of Calvin cycle in skin chloroplasts of ripe red grape berries
Description	The role of photosynthesis in fruits still challenges scientists. This is especially true in the case of mature grape berries of red varieties lined by an anthocyanin-enriched exocarp (skin) almost impermeable to gases. Although chlorophylls are degraded and replaced by carotenoids in several fruits, available evidence suggests that they may persist in red grapes at maturity. In the present study, chloroplasts were isolated from the skin of red grape berries (cv. Vinhão) to measure chlorophyll levels and the organelle proteome. The results showed that chloroplasts (and chlorophylls) are maintained in ripe berries masked by anthocyanin accumulation and that the proteome of chloroplasts from green and mature berries is distinct. Several proteins of the light reactions significantly accumulated in chloroplasts at the mature stage, including those of light-harvesting complexes of photosystems I (PSI) and II (PSII), redox chain, and ATP synthase, while chloroplasts at the green stage accumulated more proteins involved in the Calvin cycle and the biosynthesis of amino acids, including precursors of secondary metabolism. Taken together, results suggest that although chloroplasts are more involved in biosynthetic reactions in green berries, at the mature stage, they may provide ATP for energy-demanding processes such as anthocyanin biosynthesis or even O2 to feed the respiratory demand of inner tissues.
HostingRepository	PRIDE
AnnounceDate	2022-10-25
AnnouncementXML	Submission_2022-10-25_09:49:42.202.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	AntónioTeixeira
SpeciesList	scientific name: Vitis vinifera (Grape); NCBI TaxID: 29760;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-12 14:02:07	ID requested
⏵ 1	2022-10-25 09:49:42	announced

Dataset with its publication pending

submitter keyword: Grape berry skin, Chloroplasts, Photosynthesis, Vitis vinifera, Proteomics

Antónioteixeira
contact affiliation	Centre of Molecular and Environmental Biology (CBMA), Department of Biology, University of Minho, Braga, Portugal
contact email	antonio.teixeira@bio.uminho.pt
lab head
AntónioTeixeira
contact affiliation	Centre of Molecular and Environmental Biology (CBMA), Department of Biology, University of Minho, Braga, Portugal
contact email	antonio.teixeira@bio.uminho.pt
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD037346

PRIDE project URI

• PRIDE
  1. PXD037346
     1. Label: PRIDE project
     2. Name: A proteomic analysis shows stimulation of light reactions and inhibition of Calvin cycle in skin chloroplasts of ripe red grape berries