PXD037329-2

PXD037329 is an original dataset announced via ProteomeXchange.

Title	H2O2 induced oxidative stress may trigger cellular senescence in retinal photoreceptor cells through modulation of ECM-receptor interaction, oxidative phosphorylation and spliceosome pathways
Description	Photoreceptor cells are highly susceptible to oxidative stress induced damage due to their high metabolic rate. Oxidative stress plays a key role in driving pathological events in several different ocular diseases which lead to retinal degeneration and ultimately blindness. A growing number of studies have been performed to understand downstream events caused by ROS induced oxidative stress in photoreceptor cells; however, the underlying mechanisms of ROS toxicity are not fully understood. Therefore, to shed light on the ROS induced downstream pathological events, we employed a tandem mass tag (TMT) labeling-based quantitative mass-spectrometric approach to determine proteome changes in 661W photoreceptor cells following oxidative stress induction via application of different concentrations of H2O2 at different time points. Overall, 5920 proteins were identified and quantified and 450 differentially expressed proteins (DEPs) were identified, which were altered in a dose and time dependent manner in all treatment groups compared to the control group. These proteins were involved in several biological pathways including spliceosome and ribosome response, activated glutathione metabolism, decreased ECM-receptor interaction, oxidative phosphorylation, abnormally regulated lysosome, apoptosis, and ribosome biogenesis. Our results highlighted ECM-receptor interaction, oxidative phosphorylation and spliceosome pathways as the major targets of oxidative stress that might mediate vascular dysfunction and cellular senescence.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:47:40.149.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Liting Deng
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-12 08:32:24	ID requested
1	2023-05-10 14:54:22	announced
⏵ 2	2023-11-14 08:47:50	announced	2023-11-14: Updated project metadata.

Deng L, Gupta V, Abyadeh M, Chitranshi N, Pushpitha K, Wu Y, Gupta V, You Y, Paulo JA, Graham SL, Mirzaei M, Haynes PA, Oxidative Stress Induced Dysfunction of Protein Synthesis in 661W Mice Photoreceptor Cells. Proteomes, 11(2):(2023) [pubmed]

submitter keyword: Proteomics

Photoreceptor

Oxidative stress

Retina

ECM receptor interaction signaling pathway

Cellular senescence.

Paul A. Haynes
contact affiliation	School of Natural Sciences,Faculty of Science and Engineering, Macquarie University
contact email	paul.haynes@mq.edu.au
lab head
Liting Deng
contact affiliation	Macquarie University
contact email	liting.deng@hdr.mq.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD037329
     1. Label: PRIDE project
     2. Name: H2O2 induced oxidative stress may trigger cellular senescence in retinal photoreceptor cells through modulation of ECM-receptor interaction, oxidative phosphorylation and spliceosome pathways