PXD037307-1

PXD037307 is an original dataset announced via ProteomeXchange.

Title	GPI-Anchored Ligand-BioID2-Tagging System Identifies proteins Mediating Zika Virus Entry
Description	Identification of host factors facilitating pathogen entry is critical for preventing infectious diseases. Here, we report a tagging system consisting of a viral receptor-binding protein (RBP) linked to BioID2, which is expressed on the cell surface via a GPI anchor. Using VSV or Zika virus (ZIKV) RBP, the system (BioID2- RBP(V)-GPI; BioID2-RBP(Z)-GPI) faithfully identifies LDLR and AXL, the receptors of VSV and ZIKV, respectively. Being GPI-anchored is essential for the probe to function properly. Furthermore, BioID2-RBP(Z)-GPI expressed in human neuronal progenitor cells identifies galectin-1 on cell surface pivotal for ZIKV entry. This conclusion is further supported by antibody blocking and galectin-1 silencing in A549 and mouse neural cells. Importantly, Lgas1-/- mice are significantly more resistant to ZIKV infection than Lgas1+/+ littermates are, having significantly lower virus titers and fewer pathologies in various organs. This tagging system may have broad applications for identifying protein-protein interactions on the cell surface
HostingRepository	iProX
AnnounceDate	2022-10-12
AnnouncementXML	Submission_2022-10-12_01:16:10.796.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shanshan Gao
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Bruker Daltonics amaZon series

Revision	Datetime	Status	ChangeLog Entry
0	2022-10-12 01:14:59	ID requested
⏵ 1	2022-10-12 01:16:11	announced
2	2023-12-06 19:11:30	announced	2023-12-07: Update information.

Dataset with its publication pending

submitter keyword: Proximity labeling, biotin ligase, GPI anchor, lipid raft, Zika virus，Galectin-1

Chaoyang Li
contact affiliation	Affiliated Cancer Hospital and Institute of Guangzhou Medical University, State Key Laboratory of Respiratory Disease, Key Laboratory for Cell Homeostasis and Cancer Research of Guangdong High Education Institute
contact email	chaoyangli@gzhmu.edu.cn
lab head
Shanshan Gao
contact affiliation	Wuhan Institute of Virology, Chinese Academy of Sciences
contact email	gaoshanshan202207@163.com
dataset submitter

iProX dataset URI